<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Role of dorsal periaqueductal grey in instinctive defensive behavior"^^ . "To survive, animals have to finely regulate their threat assessment and escape behavior.\r\nIn mice, the brainstem dorsal periaqueductal grey (dPAG) generates innate defensive behaviors towards a multitude of threats, like predators, prey and aggressive conspecifics.\r\ndPAG acts as a switchboard between forebrain areas like the anterior cingulate cortex\r\n(ACC) and the brainstem. Glutamatergic neurons in the ACC project to dPAG and have\r\nan inhibitory effect on the structure, promoting approach. In dPAG, separate neuron ensembles correlate with risk assessment or with escape. However, the differential connectivity and gene expression of these two classes remains unknown. In this thesis, I aimed\r\nat better understand the anatomy and function of the dPAG microcircuits controlling assessment and escape behavior. To do so, I performed single neuron calcium imaging\r\nrecordings with a miniaturized fluorescent microscope in freely moving mice in glutamatergic and GABAergic neurons in dPAG, showing that both cell types correlated with assessment and escape in a social, predatorial and prey threat exposure test. Furthermore,\r\nI used volume electron microscopy combined with multiplexed labeling of pre- and\r\npostsynapses, showing that ACC axons establish synapses onto Vglut2+\r\n and non-glutamatergic, non-GABAergic neurons in dPAG, pointing to the role of a neuromodulator\r\nneuron class mediating dPAG inhibition. Further experiments will analyze the expression\r\nprofile of dPAG neurons, with a focus on ACC synaptic partners, using spatially resolved\r\ntranscriptomics, and will explore the functional role of dPAG candidate neuromodulatory\r\nneurons in defensive behaviors, such as enkephalin expressing neurons. In conclusion,\r\nmy PhD work characterized the connectivity and function of a brainstem neural circuit that\r\nmodulates innate defensive behaviors towards aggressive conspecifics, predators and\r\nprey in mice."^^ . "2024" . . . . . . . "Irene Pilar"^^ . "Ayuso Jimeno"^^ . "Irene Pilar Ayuso Jimeno"^^ . . . . . . "Role of dorsal periaqueductal grey in instinctive defensive behavior (PDF)"^^ . . . "Role of dorsal periaqueductal grey in instinctive defensive behavior (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Role of dorsal periaqueductal grey in instinctive defensive behavior (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Role of dorsal periaqueductal grey in instinctive defensive behavior (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Role of dorsal periaqueductal grey in instinctive defensive behavior (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Role of dorsal periaqueductal grey in instinctive defensive behavior (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #33961 \n\nRole of dorsal periaqueductal grey in instinctive defensive behavior\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . .