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primary topic: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation

Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation
type: Article
abstract: DNA methylome remodeling is an essential molecular mechanism underlying all stages of hematopoietic differentiation. However, current datasets only cover a fraction of the genome and are often limited to specific hematopoietic cell types. A comprehensive, genome-wide atlas of the DNA methylation dynamics during hematopoietic differentiation is still missing. Preliminary evidence suggests that the single-cell landscape of the hematopoietic stem and progenitor cell (HSPC) compartment is characterized by a structured continuum of epigenetically-defined cell states. Significant advances in charting this epigenetic state manifold have recently been achieved for the chromatin accessibility and histone modification layers. However, despite its potential importance, the landscape of single-cell DNA methylome states in the HSPC compartment remains largely unexplored. This project aimed to comprehensively map the genome-wide DNA methylation dynamics during hematopoietic differentiation and leverage this atlas as a reference to analyze the single-cell DNA methylome landscape in the HSPC compartment and among mature hematopoietic cells. The functional importance and rich information content of differentially methylated regions (DMRs) are well-established. However, the DNA methylation layer inherently possesses the capability to encode information at CpG resolution. The role and extent of differentially methylated CpG (DMCpG) programming within DMR regions is largely unexplored. This project therefore aimed to evaluate the role and mechanisms of DMCpG programming during hematopoietic differentiation. Using high-coverage tagmentation-based whole-genome bisulfite sequencing data for 25 hematopoietic populations, I have compiled a genome-wide, dual-layer DMR/DMCpG atlas, which maps, annotates, and integrates DMR and DMCpG programming during hematopoietic differentiation. Loss of stemness was associated with lineage-independent gain of DNA methylation, while lineage specification wa…
date: 2023
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
has document: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (Other)
subject: 004 Informatik
subject: 500 Naturwissenschaften und Mathematik
subject: 570 Biowissenschaften, Biologie

Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
type: Document
has file: thesis_stephen_kraemer_for_UB.pdf
has part: thesis_stephen_kraemer_for_UB.pdf

Stephen Jörg Krämer
type: Person
given name: Stephen Jörg
family name: Krämer

Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (Other)
type: Document
is version of: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
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Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (Other)
type: Document
is version of: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
is volatile version of: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
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Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (Other)
type: Document
is version of: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
is volatile version of: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
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Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (Other)
type: Document
is version of: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
is volatile version of: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
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Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (Other)
type: Document
is version of: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
is volatile version of: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
is index codes version of: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation (PDF)
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HTML Summary of #33968 Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation
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primary topic: Uncovering the mechanisms and information content of CpG-resolved DNA methylation programming during hematopoietic differentiation

004 Informatik
type: Concept
preferred label: 004 Data processing Computer science @en

500 Naturwissenschaften und Mathematik
type: Concept
preferred label: 500 Natural sciences and mathematics @en

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en