<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Analyzing the individual contributions of translation\r\ninitiation factors to shaping the proteome of cancer cells"^^ . "In living cells, proteins are synthesized through the complex multi-step process process\r\nof mRNA translation. The initiation step of translation is believed to be rate limiting step\r\nof the process and is subject to diverse regulatory mechanisms. A network of proteins,\r\nreferred to as (eukaryotic) translation initiation factors (eIF), facilitate the recruitment and\r\nactivation of the translation machinery to initiate protein synthesis. Individual eIF and protein\r\nsynthesis in general are frequently deregulated in various human cancers to enable aberrant\r\ncellular growth and proliferation. Increasing evidence suggests that individual eIF proteins\r\nregulate the translation of specific mRNA and do not simply contribute to protein synthesis\r\nglobally. Changes in protein synthesis are frequently studied using mRNA sequencing-\r\nbased approaches, however, direct measurements of newly synthesized proteins can be\r\nadvantageous. However, the majority of established proteomic methods for analyzing\r\nchanges in protein synthesis have severe limitations.\r\nIn this thesis, a new proteomics workflow was developed to simplify and improve the analysis\r\nof changes in the newly synthesized proteome across numerous conditions. This workflow\r\nwas then employed to carry out a systematic analysis of changes in protein synthesis,\r\ninduced by the depletion of 40 different eIF proteins in a human cancer cell line model.\r\nThe analysis demonstrated that depletion of different eIF lead to distinct changes in protein\r\nsynthesis, suggesting that several eIF regulate widespread preferential mRNA translation.\r\nAcquired data and findings from the research conducted in this thesis could potentially help\r\nto characterize specific signatures of eIF-mediated preferential translation of specific mRNA,\r\nwhich could aid in the development of novel therapeutic strategies.\r\nIn summary, this thesis describes an optimized proteomics workflow for the analysis of\r\nnewly synthesized proteins was developed and employed to carry out a systematic analysis\r\nof how individual eIF shape the proteome of cancer cells."^^ . "2024" . . . . . . . "Toman Deniz"^^ . "Bortecen"^^ . "Toman Deniz Bortecen"^^ . . . . . . "Analyzing the individual contributions of translation\r\ninitiation factors to shaping the proteome of cancer cells (PDF)"^^ . . . "PhD_thesis_TB.pdf"^^ . . . "Analyzing the individual contributions of translation\r\ninitiation factors to shaping the proteome of cancer cells (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Analyzing the individual contributions of translation\r\ninitiation factors to shaping the proteome of cancer cells (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Analyzing the individual contributions of translation\r\ninitiation factors to shaping the proteome of cancer cells (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Analyzing the individual contributions of translation\r\ninitiation factors to shaping the proteome of cancer cells (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Analyzing the individual contributions of translation\r\ninitiation factors to shaping the proteome of cancer cells (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #34859 \n\nAnalyzing the individual contributions of translation \ninitiation factors to shaping the proteome of cancer cells\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .