<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Modulation of Wetting and Mechanical Properties of Model Cell Membranes Caused by Changes on Molecular Levels"^^ . "Wetting is an ubiquitous phenomenon not only present in nature and biology but also\r\nof key importance in industry and medical applications. In biological systems, cell\r\nadhesion can also be understood within the framework of wetting of complex, non-\r\nNewtonian fluids. During the past several decades, physical chemistry of wetting and\r\ninterfacial interactions of biological interfaces has been developed with aid of new experimental\r\nand analytical tools. The primary aim of this thesis is to study the wetting\r\nof model cell membranes by using the combination of various physicochemical techniques.\r\nIn section 4 the wetting (adhesion) of bio-inspired, stimulus responsive polymer\r\nbrushes with simple model cells, giant vesicles,were studied. First, the switching\r\nof polymer brush conformation was monitored by using high energy specular X-ray\r\nreflectivity. Then, the global shape of giant vesicles at relaxed and compacted states\r\nwere monitored by confocal fluorescence microscopy. As the shape of vesicles is not\r\ndetermined by the surface tension (like the case of Newtonian fluid), the shape (height\r\nprofile) of vesicle near the surface, dominated by the membrane elasticity, was analyzed\r\nby microinterferometry. This enables the quantitative calculation of the adhesion\r\nfree energy of vesicles. Moreover, owing to the capability of the microinterferometry to\r\ndetermine the height fluctuation in nm accuracy, the interfacial potential between the\r\npolymer brush and the giant vesicle could be calculated quantitatively. This line of study\r\nwas further extended in section 5, where the dynamic switching of the vesicle-brush interaction\r\nwas monitored. The transition between from non-wetting (off) to wetting (on),\r\nand vice versa, was was monitored in real time by the combination of microfluidics and\r\nmicrointerferometry with the time resolution of 30 ms. It is well known that not only\r\nthe adhesion but also the binding of proteins changes the mechanical properties of\r\nlipid membranes. In section 6, the influence of subtle changes in molecular structures\r\non the protein-membrane interaction and hence the membrane mechanics was investigated\r\nusing the same membrane model (giant vesicles). As a biologically relevant\r\nmodel, the modulation of the membrane mechanics by the binding of the acute phase\r\ninflammatory C-reactive protein (CRP) to the vesicles containing oxidized lipids was investigated.\r\nFirst, the potential role of electrostatic interaction was studied by measuring\r\nthe zeta potential of lipid vesicles, suggesting that CRP binds to the vesicles containing\r\nlipids with a higher oxidative level. In the next step, the influence of CRP binding on\r\nthe mechanical properties of the membranes was calculated from the Fourier analysis\r\nof the membrane fluctuation, indicating that the binding of CRP to highly oxidized lipids\r\ncaused the most prominent change in the bending rigidity of the membrane. The\r\nobtained results demonstrated how the subtle changes on the molecular level, such as\r\nthe compaction of polymer brushes and the oxidation of lipids, significantly modulates\r\nthe wetting and mechanical properties of lipid membranes."^^ . "2024" . . . . . . . "Felix"^^ . "Weißenfeld"^^ . "Felix Weißenfeld"^^ . . . . . . "Modulation of Wetting and Mechanical Properties of Model Cell Membranes Caused by Changes on Molecular Levels (PDF)"^^ . . . "Dissertation_Felix-Weißenfeld_2024.pdf"^^ . . . "Modulation of Wetting and Mechanical Properties of Model Cell Membranes Caused by Changes on Molecular Levels (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Modulation of Wetting and Mechanical Properties of Model Cell Membranes Caused by Changes on Molecular Levels (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Modulation of Wetting and Mechanical Properties of Model Cell Membranes Caused by Changes on Molecular Levels (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Modulation of Wetting and Mechanical Properties of Model Cell Membranes Caused by Changes on Molecular Levels (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Modulation of Wetting and Mechanical Properties of Model Cell Membranes Caused by Changes on Molecular Levels (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #34869 \n\nModulation of Wetting and Mechanical Properties of Model Cell Membranes Caused by Changes on Molecular Levels\n\n" . "text/html" . . . "540 Chemie"@de . "540 Chemistry and allied sciences"@en . .