title: An end-to-end pipeline to study and  model proton radiation-induced effects  of the central nervous system as a  function of dose and linear energy  transfer at the single-cell level in 3D
creator: Boucsein, Marc Oliver
subject: ddc-530
subject: 530 Physics
description: Proton therapy is a promising form of brain radiotherapy due to its characteristic  Bragg peak profile sparing normal tissue. However, late side effects have been observed  and the underlying mechanisms are not fully understood. An end-to-end pipeline  was first proposed to investigate late brain tissue-specific radiation-induced effects  associated with the central nervous system (CNS) as a function of dose and linear  energy transfer (LET) at the single-cell level in 3D for a well-established preclinical  experiment mimicking patient treatment. This pipeline includes Monte Carlo-based  dose and LET calculation, target delivery verification, image processing tasks in-  cluding preprocessing, local-affine multi-step registrations, a topology-preserving  segmentation, and analysis modules using 2-point density correlation functions, the  Riemann elastic metric, and the CNS network size to measure compactness, shape  and size heterogeneity. As a proof of principle, the pipeline was applied to investigate  the observed radiation-induced astrogliosis segmented with a Dice coefficient of 0.86,  achieving a maximum dose and LET uncertainty of 2.5 Gy and 2 keV/mum. In  conclusion, the analysis reveals that the process of astrogliosis can be considered as  a functional acting unit showing a radiation-induced late effect as a function of the  brain tissue type, dose and LET indicating a variable relative biological effectiveness.
date: 2024
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/35224/1/PhD_Thesis_Marc_Boucsein.pdf
identifier: DOI:10.11588/heidok.00035224
identifier: urn:nbn:de:bsz:16-heidok-352243
identifier:   Boucsein, Marc Oliver  (2024) An end-to-end pipeline to study and model proton radiation-induced effects of the central nervous system as a function of dose and linear energy transfer at the single-cell level in 3D.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/35224/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng