<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> .
<> <http://xmlns.com/foaf/0.1/primaryTopic> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://purl.org/dc/terms/title> "Insights into Autoimmune Mechanisms and the Pathogenic Role of Tumor-Infiltrating B Cells in Yo-Associated Paraneoplastic Cerebellar Degeneration"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://purl.org/ontology/bibo/abstract> "Paraneoplastic cerebellar degeneration (PCD) is an autoimmune neurological disorder that impairs motor function through the immune-mediated death of cerebellar neurons. PCD is frequently diagnosed among ovarian or breast cancer patients, where the tumor aberrantly expresses antigens typically restricted to the cerebellum. Yo-associated PCD is the most common subtype, characterized by serum- or cerebrospinal fluid (CSF)-derived Yo antibodies. They serve \r\nas a diagnostic marker, and prompts the search for an associated tumor. Yo antibodies have been \r\nclassically defined to target the canonical Yo antigen Cerebellar Degeneration-related protein 2 \r\n(CDR2), whose expression is normally restricted to Purkinje cells of the cerebellum. Much \r\ncontention however exists surrounding the specificity of these autoantibodies and their \r\ncontribution to the progression of the disorder. In my dissertation, I used single-cell \r\nimmunoglobulin sequencing to examine the repertoire of tumor-infiltrating B cells and antibody�secreting cells from an ovarian carcinoma patient who presented with anti-Yo paraneoplastic \r\ncerebellar degeneration (Yo-PCD). Here, I show across different reactivity assays that CDR2L \r\n(Cerebellar Degeneration Related Protein 2 Like), is also a target of Yo antibodies, and may be \r\nthe predominant Yo antigen. I also show that these Yo antibodies are secreted by highly-activated \r\nand somatically hypermutated antibody-secreting cells that infiltrate the tumor, and likely derive \r\nfrom protracted autoreactive germinal center responses. I could also show that these Yo \r\nantibodies can be taken up by Purkinje cells in vitro. Finally, I could show that after continuous \r\nintrathecal delivery of monoclonal Yo antibodies in mice, they are also taken up by Purkinje cells \r\nin vivo, and normal motor function, evaluated through rotarod assays, is affected. My results \r\ndemonstrate that tumor-derived Yo antibodies mainly target CDR2L, and have a potential \r\npathogenic contribution to the progression of Yo-PCD. Resolving the relevant Yo antigen and \r\ndissecting the epitope specificities of Yo autoantibodies may help improve the diagnosis of this \r\ndisorder, and the early detection of the associated tumor. Furthermore, deeply characterizing Yo \r\nautoantibodies and elucidating antibody-mediated pathogenic mechanisms may fine-tune the \r\nprognosis for Yo-PCD, and expand current paradigms for the role of antibodies targeting \r\nintracellular antigens"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://purl.org/dc/terms/date> "2025" .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://www.w3.org/2002/07/owl#sameAs> <https://doi.org/10.11588/heidok.00035284> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://purl.org/dc/terms/creator> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/person/ext-f427ccdc24867e250abcf80d628b327c> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://purl.org/ontology/bibo/authorList> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284#authors> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_1> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/person/ext-f427ccdc24867e250abcf80d628b327c> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/person/ext-f427ccdc24867e250abcf80d628b327c> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/person/ext-f427ccdc24867e250abcf80d628b327c> <http://xmlns.com/foaf/0.1/givenName> "Joshua Reginald"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/person/ext-f427ccdc24867e250abcf80d628b327c> <http://xmlns.com/foaf/0.1/familyName> "Malapit"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/person/ext-f427ccdc24867e250abcf80d628b327c> <http://xmlns.com/foaf/0.1/name> "Joshua Reginald Malapit"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/EPrint> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/DoctoralThesisEPrint> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://purl.org/dc/terms/isPartOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/repository> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://eprints.org/ontology/hasDocument> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> <http://www.w3.org/2000/01/rdf-schema#label> "Insights into Autoimmune Mechanisms and the Pathogenic Role of Tumor-Infiltrating B Cells in Yo-Associated Paraneoplastic Cerebellar Degeneration (PDF)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://eprints.org/ontology/hasDocument> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258281> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258281> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258281> <http://www.w3.org/2000/01/rdf-schema#label> "Insights into Autoimmune Mechanisms and the Pathogenic Role of Tumor-Infiltrating B Cells in Yo-Associated Paraneoplastic Cerebellar Degeneration (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258281> <http://eprints.org/relation/isVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258281> <http://eprints.org/relation/isVolatileVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258281> <http://eprints.org/relation/isIndexCodesVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://eprints.org/ontology/hasDocument> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258282> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258282> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258282> <http://www.w3.org/2000/01/rdf-schema#label> "Insights into Autoimmune Mechanisms and the Pathogenic Role of Tumor-Infiltrating B Cells in Yo-Associated Paraneoplastic Cerebellar Degeneration (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258282> <http://eprints.org/relation/isVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258282> <http://eprints.org/relation/isVolatileVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258282> <http://eprints.org/relation/islightboxThumbnailVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://eprints.org/ontology/hasDocument> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258283> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258283> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258283> <http://www.w3.org/2000/01/rdf-schema#label> "Insights into Autoimmune Mechanisms and the Pathogenic Role of Tumor-Infiltrating B Cells in Yo-Associated Paraneoplastic Cerebellar Degeneration (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258283> <http://eprints.org/relation/isVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258283> <http://eprints.org/relation/isVolatileVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258283> <http://eprints.org/relation/ispreviewThumbnailVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://eprints.org/ontology/hasDocument> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258284> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258284> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258284> <http://www.w3.org/2000/01/rdf-schema#label> "Insights into Autoimmune Mechanisms and the Pathogenic Role of Tumor-Infiltrating B Cells in Yo-Associated Paraneoplastic Cerebellar Degeneration (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258284> <http://eprints.org/relation/isVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258284> <http://eprints.org/relation/isVolatileVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258284> <http://eprints.org/relation/ismediumThumbnailVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://eprints.org/ontology/hasDocument> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258285> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258285> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258285> <http://www.w3.org/2000/01/rdf-schema#label> "Insights into Autoimmune Mechanisms and the Pathogenic Role of Tumor-Infiltrating B Cells in Yo-Associated Paraneoplastic Cerebellar Degeneration (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258285> <http://eprints.org/relation/isVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258285> <http://eprints.org/relation/isVolatileVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258285> <http://eprints.org/relation/issmallThumbnailVersionOf> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/document/258280> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://www.w3.org/2000/01/rdf-schema#seeAlso> <https://archiv.ub.uni-heidelberg.de/volltextserver/35284/> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/35284/> <http://purl.org/dc/elements/1.1/title> "HTML Summary of #35284 \n\nInsights into Autoimmune Mechanisms and the Pathogenic Role of Tumor-Infiltrating B Cells in Yo-Associated Paraneoplastic Cerebellar Degeneration\n\n" .
<https://archiv.ub.uni-heidelberg.de/volltextserver/35284/> <http://purl.org/dc/elements/1.1/format> "text/html" .
<https://archiv.ub.uni-heidelberg.de/volltextserver/35284/> <http://xmlns.com/foaf/0.1/primaryTopic> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/subject/ddc-570> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://www.w3.org/2004/02/skos/core#Concept> .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/subject/ddc-570> <http://www.w3.org/2004/02/skos/core#prefLabel> "570 Biowissenschaften, Biologie"@de .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/subject/ddc-570> <http://www.w3.org/2004/02/skos/core#prefLabel> "570 Life sciences"@en .
<https://archiv.ub.uni-heidelberg.de/volltextserver/id/eprint/35284> <http://purl.org/dc/terms/subject> <https://archiv.ub.uni-heidelberg.de/volltextserver/id/subject/ddc-570> .