<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Protein-RNA interactions in centromeric chromatin"^^ . "Chromosome segregation is a complex and tightly regulated process that is necessary for \r\nproper cell division. Chromosomes are aligned at the equatorial plate and transported to \r\nnascent daughter cells using motor proteins and microtubules. They are attached to the \r\nmicrotubules by a multiprotein complex, the kinetochore. Kinetochores are anchored to\r\nthe centromeric regions of the chromosome. These regions consist of repetitive noncoding \r\nDNA which forms the centromeric heterochromatin and is embedded in larger regions of \r\nnoncoding pericentromeric heterochromatin. Centromeres are not defined by an \r\nunderlying DNA sequence, but rather by the epigenetic marker Cenp-A, a histone H3 \r\nvariant specific for centromeres. \r\nCenp-A, or Cid in Drosophila melanogaster (fruit fly), together with its loading factor \r\nCal1 and the Cenp-C protein form the basis of the inner kinetochore complex. The outer \r\nkinetochore then connects to the spindle microtubule. This process requires the presence \r\nof noncoding RNAs. These RNAs are transcribed from the pericentromeric regions and \r\ncolocalise with the inner parts of the kinetochore. The absence of these RNAs leads to \r\nchromosome segregation defects, but their exact function as well as their interaction \r\npartners are not well described. These defects are observed not just in Drosophila \r\nmelanogaster, but also in every other organism that has been researched to date. \r\nIn this work, I investigated the interaction of centromeric proteins of Drosophila \r\nmelanogaster with centromeric RNA using biochemical assays (EMSA) and mass \r\nspectrometry (HDX-MS). I overexpressed and purified proteins as well as in vitro\r\ntranscribed RNAs. I observed the interaction of kinetochore proteins and centromeric \r\nRNA with focus on Cal1 and satellite RNA. I demonstrated that Cal1 fragments are \r\nRNA-binding using EMSA. However, HDX-MS was not sufficient to measure the details \r\nof the interaction, as addition of RNA did not significantly change the deuteration pattern \r\nof the protein fragments. I observed that Cal1 is unfolded and the fragment I worked with \r\nis a promiscuous RNA-binder."^^ . "2024" . . . . . . . "Vojtech"^^ . "Dolejs"^^ . "Vojtech Dolejs"^^ . . . . . . "Protein-RNA interactions in centromeric chromatin (PDF)"^^ . . . "Dissertation Dolejš.pdf"^^ . . . "Protein-RNA interactions in centromeric chromatin (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Protein-RNA interactions in centromeric chromatin (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Protein-RNA interactions in centromeric chromatin (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Protein-RNA interactions in centromeric chromatin (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Protein-RNA interactions in centromeric chromatin (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #35344 \n\nProtein-RNA interactions in centromeric chromatin\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .