<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "The role of the actin-remodeling proteins L-plastin and cofilin-1 in mast cells and B cells"^^ . "Various processes in immune cells require regulated actin cytoskeleton remodeling which is mediated by a plethora of proteins. The functions of the actin-remodeling proteins cofilin-1 (Cfl1) and L-plastin (LPL) in B cells and mast cells have not been fully elucidated so far.\r\nThe actin-severing and -depolymerizing protein Cfl1 is ubiquitously expressed in non-muscle cells including immune cells. Functional Cfl1 is essential for αβ T cell development and T cell costimulation. One aim of this thesis was to find out whether Cfl1 expression is also required for normal B cell and mast cell organization. Mice expressing non-functional Cfl1 mast cell-specifically were devoid of connective tissue mast cells (CTMCs) and did not develop systemic anaphylaxis. Thus, they are a suitable model for CTMC deficiency. CTMC-deficient mice showed normal reactions in in vivo models for allergic contact dermatitis, psoriasis, and clearing vaccinia virus-induced skin infection demonstrating no major impact of CTMCs in these disease models. Mice with B cell-specific expression of non-functional Cfl1 had drastically diminished B cell numbers in spleen, lymph nodes, blood, and peritoneum. Also, in the bone marrow the percentage of B cells was reduced. Residual B cells were shown to have an imperfect knock-in of non-functional Cfl1. In summary, expression of non-functional Cfl1 instead of the wildtype protein specifically in B cells or mast cells revealed a fundamental role of functional Cfl1 for the normal appearance of B cells and mast cells in mice.\r\nThe leukocyte-specific actin-bundling protein LPL is involved in several processes important for immunity, such as T cell motility and activation. LPL phosphorylation at serine-5 (S5) can increase its actin-binding and -bundling activity. In order to clarify the impact of LPL-S5 phosphorylation for B cells and mast cells, knock-in mouse lines expressing a non-phosphorylatable LPL form instead of the wildtype protein in B cells or mast cells were characterized in this thesis. In the mutant protein S5 was exchanged by non-phosphorylatable alanine (LPL-S5A). LPL-S5A expression in CTMCs led to a diminished peritoneal mast cell population. However, these mice showed a normal reaction in the in vivo model for systemic anaphylaxis. Mice expressing LPL-S5A B cell-specifically possessed a decreased splenic B cell number. The number of follicular B cells was moderately diminished while marginal zone B cells were nearly absent. In vitro experiments revealed an important role of LPL phosphorylation for chemokine-mediated migration of splenic B cells. The generated mouse lines allow to investigate whether LPL phosphorylation could be a leukocyte-specific target for immune modulation."^^ . "2025" . . . . . . . "Johanna"^^ . "Kramer"^^ . "Johanna Kramer"^^ . . . . . . "The role of the actin-remodeling proteins L-plastin and cofilin-1 in mast cells and B cells (PDF)"^^ . . . "The role of the actin-remodeling proteins L-plastin and cofilin-1 in mast cells and B cells (Other)"^^ . . . . . . "The role of the actin-remodeling proteins L-plastin and cofilin-1 in mast cells and B cells (Other)"^^ . . . . . . "The role of the actin-remodeling proteins L-plastin and cofilin-1 in mast cells and B cells (Other)"^^ . . . . . . "The role of the actin-remodeling proteins L-plastin and cofilin-1 in mast cells and B cells (Other)"^^ . . . . . . "The role of the actin-remodeling proteins L-plastin and cofilin-1 in mast cells and B cells (Other)"^^ . . . . . "HTML Summary of #35509 \n\nThe role of the actin-remodeling proteins L-plastin and cofilin-1 in mast cells and B cells\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .