<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "An interaction network of luminal centrosomal proteins\r\norganized by POC1A and POC1B ensures centriolar integrity"^^ . "The centrosome is a membrane-less organelle and in eukaryotes the main microtubuleorganizing\r\ncenter (MTOC). Due to its ability to nucleate and organize microtubules (MT), it\r\nplays an important role in cell division, ciliogenesis, cell polarity and cell migration. Therefore,\r\nit is not surprising that disfunction of centrosomes has devastating consequences for an\r\norganism, leading to various diseases ranging from cancer and ciliopathies to mental and\r\nbehavioural disorders. The centriole, as the basic structure of the centrosome, is crucial to\r\nensure centrosomal function. Structural defects of centrioles have direct consequences on\r\ncentrosome function. One factor contributing to the centriolar stability is the inner scaffold, a\r\nring-like substructure in the lumen of the central-to-distal half of the centriole. Based on their\r\nlocalization, POC1B (proteome of centriole 1B), POC5, FAM161A and CCDC15 are suggested\r\ninner scaffold components, however, the relationship between these proteins and if other\r\nproteins are part of the inner scaffold is still unclear.\r\nHere, I identified POC1A, the human paralogue of POC1B, as a novel inner scaffold component\r\nand investigated the function of the two human POC1 proteins within the inner scaffold. Both\r\nPOC1 proteins are inner centriole proteins with overlapping localization. However, while\r\nPOC1A resides closer towards the centriole lumen, POC1B is in proximity to the centriole wall.\r\nLoss of POC1A or POC1B affects the centriolar localization of inner scaffold components like\r\nPOC5, FAM161A, Centrin and CCDC15. Based on the results presented in this study, POC1APOC1B\r\nheterodimers organize the complex protein network of the inner scaffold by crosslinking\r\ndifferent proteins. This is achieved by the ability to interact with various proteins\r\nthrough different interaction modes mediated by their N-terminal WD40 domain, C-terminal\r\ncoiled-coil region, or both. Crucial for the inner scaffold is the interaction between POC1A and\r\nPOC5, and the ability of POC5 to form a tetramer. POC1A-POC1B heterodimers interact with\r\nthe MT-binding proteins FAM161A and MDM1, which may lead to a positioning of the POC5\r\ntetramer close to the centriolar wall. Disruption of the inner scaffold leads to broken centrioles\r\nand mitotic defects, confirming the importance of the inner scaffold in maintaining centriole\r\nintegrity. In addition, this study shows that combined loss of POC1A and POC1B results in\r\ncomplete disintegration of centrioles, highlighting their role in centriole biogenesis and\r\nstability."^^ . "2025" . . . . . . . "Cornelia"^^ . "Sala"^^ . "Cornelia Sala"^^ . . . . . . "An interaction network of luminal centrosomal proteins\r\norganized by POC1A and POC1B ensures centriolar integrity (PDF)"^^ . . . "PhD_Thesis_Cornelia_Sala.pdf"^^ . . . "An interaction network of luminal centrosomal proteins\r\norganized by POC1A and POC1B ensures centriolar integrity (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "An interaction network of luminal centrosomal proteins\r\norganized by POC1A and POC1B ensures centriolar integrity (Other)"^^ . . . . . . "preview.jpg"^^ . . . "An interaction network of luminal centrosomal proteins\r\norganized by POC1A and POC1B ensures centriolar integrity (Other)"^^ . . . . . . "medium.jpg"^^ . . . "An interaction network of luminal centrosomal proteins\r\norganized by POC1A and POC1B ensures centriolar integrity (Other)"^^ . . . . . . "small.jpg"^^ . . . "An interaction network of luminal centrosomal proteins\r\norganized by POC1A and POC1B ensures centriolar integrity (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #36438 \n\nAn interaction network of luminal centrosomal proteins \norganized by POC1A and POC1B ensures centriolar integrity\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .