<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Reshape of CAR-T cells by overexpression of TCF-1"^^ . "Despite the remarkable success of CAR-T cell therapy in leukemia and lymphoma patients, lack of CAR-T cell persistence remains a significant clinical problem. In particular, CAR-T cells often exhibit a deficiency in TCF-1 expression, rendering cells susceptible to TCF-1-associated pathways and consequently to short survival in the host. Therefore, it is intriguing to modulate TCF-1 expression in CAR-T cells to address the challenge of CAR-T cell persistence and further improve therapeutic outcomes.\r\nIn this study, third generation CAR vectors were used, and a double transduction system was established. The effect of TCF-1 overexpression in CAR-T cells was comprehensively explored regarding various aspects. Given the phenotype and functionality of CAR-T cells after transduction, two different CAR models (CD19.CAR vector and CD33.CAR vector) were settled in the study to generalize the effect of overexpression of TCF-1. We found that (1) the overexpression of TCF-1 can enrich the naïve and stem cell-like CAR-T cells exhibiting a better killing efficacy; (2) the cytokine profile of CAR-T cells were switched from proinflammatory or killing related to inactive through overexpression of TCF-1; (3) the apoptotic status of CAR- T cells could be modulated by TCF-1 overexpression, thus resulting in a better long- term functionality in terms of repetitive tumor killing and reduced multiple inhibitory receptors.\r\nIn summary, overexpression of TCF-1 in CAR-T cells could reduce apoptosis, occurrence of CRS and improve their persistence, resulting in enhanced antitumor efficacy of CAR-T cells. Thus, overexpression of TCF-1 might improve CAR-T cell persistence in vivo and enhance the effect of CAR-T cell therapy in patients with relapsed/refractory hematologic malignancies."^^ . "2025" . . . . . . . "Hao"^^ . "Yao"^^ . "Hao Yao"^^ . . . . . . "Reshape of CAR-T cells by overexpression of TCF-1 (PDF)"^^ . . . "Yao_Hao_1992_12_28_Reshape of CAR_T cells by overexpression of TCF_1.pdf"^^ . . . "Reshape of CAR-T cells by overexpression of TCF-1 (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Reshape of CAR-T cells by overexpression of TCF-1 (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Reshape of CAR-T cells by overexpression of TCF-1 (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Reshape of CAR-T cells by overexpression of TCF-1 (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Reshape of CAR-T cells by overexpression of TCF-1 (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #36449 \n\nReshape of CAR-T cells by overexpression of TCF-1\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . .