<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Engineered Models of Human Microvasculature: Investigating the Cardiac Microenvironment’s Role in Vascular \r\nRemodeling"^^ . "Studying the myocardial microvasculature has been challenging, in part, due to technical \r\nimaging limitations in vivo and limited physiologically relevant in vitro models. This thesis reports \r\nthe development of two on-chip models of human microvasculature to study the impact of the tissue \r\nmicroenvironment on cardiac vascular remodeling—with a specific focus on the contributions of \r\nstromal cells, cardiomyocytes, and female sex hormones in regulating microvascular behavior. First, \r\nthe effect of vascular-stromal crosstalk was studied by assessing the impact of tissue-specific co-\r\ncultured fibroblasts on microvascular development. The results presented herein, demonstrate that \r\nfibroblasts impact microvascular morphology by increasing branch density while decreasing vessel \r\ndiameter. Cardiac fibroblasts, unlike lung fibroblasts, cause a reduction in vascular barrier function. \r\nMoreover, lung and cardiac fibroblasts had a differential response to TGFβ1, mimicking different \r\naspects of fibrosis on-chip. This published first work highlighted the importance of fibroblasts in \r\ndeveloping tissue-specific models for vascular research. Next, a 3D model that mimics coronary \r\nmicrovascular structure and barrier function was developed to explore vascular-myocyte crosstalk. \r\nHuman-induced pluripotent stem cell (hiPSC)-derived cardiac spheroids (CS) were co-cultured with \r\ncardiac microvasculature to assess the impact of myocyte crosstalk on microvessel development. \r\nCardiomyocytes altered vessel morphology and improved barrier function in a spatially dependent \r\nmanner. This vascularized cardiac model was used to investigate the cardioprotective roles of the sex \r\nhormones estrogen (E2) and progesterone (P4) under inflammatory conditions. Pre-treatment with \r\nthese hormones preserves endothelial barrier integrity during TNFα-induced inflammation, potentially \r\nby modulating endothelin-1 secretion. Overall, these models highlight the critical role of cellular \r\ncrosstalk and the tissue microenvironment in vascular development and showcase their utility in \r\nmodeling vascular perturbations."^^ . "2026" . . . . . . . "Akinola"^^ . "Akinbote"^^ . "Akinola Akinbote"^^ . . . . . . "Engineered Models of Human Microvasculature: Investigating the Cardiac Microenvironment’s Role in Vascular \r\nRemodeling (PDF)"^^ . . . "Engineered Models of Human Microvasculature: Investigating the Cardiac Microenvironment’s Role in Vascular \r\nRemodeling (Other)"^^ . . . . . . "Engineered Models of Human Microvasculature: Investigating the Cardiac Microenvironment’s Role in Vascular \r\nRemodeling (Other)"^^ . . . . . . "Engineered Models of Human Microvasculature: Investigating the Cardiac Microenvironment’s Role in Vascular \r\nRemodeling (Other)"^^ . . . . . . "Engineered Models of Human Microvasculature: Investigating the Cardiac Microenvironment’s Role in Vascular \r\nRemodeling (Other)"^^ . . . . . . "Engineered Models of Human Microvasculature: Investigating the Cardiac Microenvironment’s Role in Vascular \r\nRemodeling (Other)"^^ . . . . . "HTML Summary of #36468 \n\nEngineered Models of Human Microvasculature: Investigating the Cardiac Microenvironment’s Role in Vascular \nRemodeling\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . . . "600 Technik, Medizin, angewandte Wissenschaften"@de . "600 Technology (Applied sciences)"@en . . . "610 Medizin"@de . "610 Medical sciences Medicine"@en . . . "620 Ingenieurwissenschaften"@de . "620 Engineering and allied operations"@en . .