<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Organellar remodeling during stress in Saccharomyces cerevisiae"^^ . "Cells have to adapt rapidly to changing physiological demands, such as differentiation, stress\r\nand disease. Extensive studies have defined many stress response pathways at the level of\r\ngene activity and protein abundance, but stress-induced changes of protein subcellular\r\nlocalization have not been investigated comprehensively on a proteome-wide level.\r\nIn this thesis, I established and applied the Dynamic Organellar Maps (DOMs) approach by\r\nlabel-free mass spectrometry for the first time to yeast. DOMs detected protein localization\r\nchanges of native, untagged proteins upon different stresses.\r\nFirstly, I used endoplasmic reticulum (ER) stress and found that almost 10% of the known\r\nproteins expressed in yeast, shifted within or between organelles. I showed that a distinct\r\nmultitude of secretory pathway proteins accumulated in the ER. In-depth analysis and\r\nexperimental validation refined the extent and selectivity of misfolded proteins retained in\r\nthe ER. Moreover, I identified new ER proteins subjected to the ER reflux pathway and\r\nidentified transmembrane components of reticulon clusters, which separated from the\r\nresidual ER. Lastly, I demonstrated that nuclear pore complex integrity was altered which\r\naffected nuclear import.\r\nSecondly, I applied DOMs to nitrogen-starved cells to identify novel cargo proteins which were\r\ndegraded by non-selective macroautophagy. I detected several proteins which were already\r\nknown to be autophagic cargos or to be involved in cargo recruitment and transport. Further\r\nanalysis provided a list of candidate cargo proteins which serve as a basis to determine if\r\nstarvation induced autophagy degrades cargo proteins non-selectively.\r\nIn conclusion, I established DOMs in yeast and revealed that global and suborganellar changes\r\nin protein localization are key elements of cellular stress responses."^^ . "2025" . . . . . . . "Anna"^^ . "Platzek"^^ . "Anna Platzek"^^ . . . . . . "Organellar remodeling during stress in Saccharomyces cerevisiae (PDF)"^^ . . . "PhD Thesis_Platzek.pdf"^^ . . . "Organellar remodeling during stress in Saccharomyces cerevisiae (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Organellar remodeling during stress in Saccharomyces cerevisiae (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Organellar remodeling during stress in Saccharomyces cerevisiae (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Organellar remodeling during stress in Saccharomyces cerevisiae (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Organellar remodeling during stress in Saccharomyces cerevisiae (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #36746 \n\nOrganellar remodeling during stress in Saccharomyces cerevisiae\n\n" . "text/html" . . . "500 Naturwissenschaften und Mathematik"@de . "500 Natural sciences and mathematics"@en . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .