…
comment: The repository administrator has not yet configured an RDF license.
primary topic: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells

From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells
type: Article
abstract: Oligodendrocyte lineage cells (OLCs) are key contributors to the development and maintenance of healthy neural circuits in the brain. They begin their life as oligodendrocyte precursor cells (OPCs), which form synapses with neurons and actively monitor their activity, before transforming into premyelinating oligodendrocytes (pmOLs) and eventually mature oligodendrocytes (OLs), the cells responsible for myelination in the central nervous system (CNS). Crucially, OLCs maintain the ability to proliferate and generate new OLs throughout adulthood, a process that is essential to sustain adaptive myelination and myelin repair. While the proper development of OLCs is vital to the overall function of brain circuits, little is known to date about the specific mechanisms that govern their evolution, including if and how neuronal activity dictates their fate and survival. In this study, I explore the role of Ca2+, an all-important second messenger, across the entire oligodendrocyte lineage and investigate its impact on OLCs at both the cellular and population level. Using state-of-the art multiphoton microscopy and novel transgenic mouse lines, I performed chronic Ca2+ imaging of OLCs in the cortex of awake “freely-moving” mice to simultaneously monitor intracellular Ca2+ dynamics and lineage progression. I found that OLCs produce Ca2+ transients that vary substantially both spatially (ranging from micrometer-size Ca2+ microdomains to branch-wide and somatic events) and temporally (ranging from hundreds of milliseconds to over 10 seconds) and that they exhibit distinct Ca2+ signatures depending on their maturity level as well as on the arousal state of the animal itself. In addition, I discovered that norepinephrine (NE), which is released as mice engage in exploratory behavior, significantly impacts OLC Ca2+ dynamics and promotes their differentiation into mature OLs. I also show that these mature OLs are capable of transducing mechanical stimuli into Ca2+ signals, which coul…
date: 2026-05-01
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
has document: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (Other)
subject: 500 Naturwissenschaften und Mathematik
subject: 570 Biowissenschaften, Biologie

From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
type: Document

Frederic Fiore
type: Person
given name: Frederic
family name: Fiore

From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (Other)
type: Document
is version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
is volatile version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
islightbox thumbnail version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)

From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (Other)
type: Document
is version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
is volatile version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
ispreview thumbnail version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)

From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (Other)
type: Document
is version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
is volatile version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
ismedium thumbnail version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)

From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (Other)
type: Document
is version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
is volatile version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
issmall thumbnail version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)

From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (Other)
type: Document
is version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
is volatile version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)
is index codes version of: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells (PDF)

HTML Summary of #37466 From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells
format: text/html
primary topic: From Signal Diversity to Cellular Identity – How Complex Ca2+ Transients Shape the Fate of Oligodendrocyte Lineage Cells

500 Naturwissenschaften und Mathematik
type: Concept
preferred label: 500 Natural sciences and mathematics @en

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en