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primary topic: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito

Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito
type: Article
abstract: Malaria, caused by Plasmodium parasites, remains one of the most devastating infectious diseases globally, with transmission relying on the parasite’s complex life cycle between mosquito vectors and mammalian hosts. The sporozoite, the parasite’s transmissive stage, is a highly motile, crescent-shaped cell whose journey from the mosquito midgut to the salivary glands is essential for host infection. Sporozoite motility, egress, and invasion are mediated by surface proteins, among which Thrombospondin-related protein 1 (TRP1) plays a critical role. In this study, I dissected the functional contributions of distinct TRP1 domains in Plasmodium berghei using genetic and molecular approaches. C-terminal deletion and domain-swap mutants revealed that the TRP1 C-terminus is not required for oocyst egress, contrary to previous reports, but is essential for productive sporozoite motility and salivary gland invasion. Functional specificity was further demonstrated as the P. berghei TRAP C-terminal domain could not substitute for TRP1, whereas the shorter P. falciparum TRP1 C-terminus fully restored function. Mutational analyses of the N-terminal Thrombospondin repeat (TSR) domain identified conserved tryptophan residues as critical for sporozoite motility and salivary gland invasion, with TRAP TSR unable to compensate for TRP1 TSR function. Dual-tagging and fluorescent labeling of TRP1 provided novel insights into its localization and domain dynamics within sporozoites. Furthermore, proximity-dependent biotinylation using TRP1-APEX identified 307 potential interacting proteins, including nine uncharacterized candidates highly expressed during mosquito stages, suggesting a broader molecular network supporting sporozoite motility and host transmission. Collectively, this work establishes TRP1 as an indispensable regulator of sporozoite motility and salivary gland invasion, with highly specific domain functions, and identifies candidate interaction partners for future mechanisti…
date: 2025
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
has document: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (Other)
subject: 570 Biowissenschaften, Biologie

Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
type: Document
has file: 16052025 PhD Thesis Monami Roy Chowdhury.pdf
has part: 16052025 PhD Thesis Monami Roy Chowdhury.pdf

Monami Roy Chowdhury
type: Person
given name: Monami
family name: Roy Chowdhury

Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (Other)
type: Document
is version of: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
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Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (Other)
type: Document
is version of: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
is volatile version of: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
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Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (Other)
type: Document
is version of: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
is volatile version of: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
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Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (Other)
type: Document
is version of: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
is volatile version of: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
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Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (Other)
type: Document
is version of: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
is volatile version of: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito (PDF)
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primary topic: Studying the role of Thrombospondin-related protein 1 (TRP1) in sporozoite motility and its journey through the mosquito

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en