eprintid: 4012 rev_number: 31 eprint_status: archive userid: 1 dir: disk0/00/00/40/12 datestamp: 2003-01-06 13:55:53 lastmod: 2012-08-14 15:09:48 status_changed: 2012-08-14 15:09:48 type: report metadata_visibility: show creators_name: Wegmann, Dominik creators_name: Hess, Pablo creators_name: Baier, Carola creators_name: Wieland, Felix T. creators_name: Reinhard, Constanze title: Supplemental Materials: Novel Isotypic g/z-Subunits Reveal Three Coatomer Complexes in Mammals ispublished: pub subjects: 570 divisions: 711000 cterms_swd: Golgi cterms_swd: COPI vesicles cterms_swd: coatomer isotypes cterms_swd: secretory pathway cterms_swd: g2-COP and z2-COP abstract: Fig. A 1 shows a Western blot analyzing the specificity of the antibodies against g- and z-isotypes. These antibodies were probed in different mammalian species (Fig. A 2). Coatomer was labeled with [35S]-methionine for different time periods, and the incorporation of [35S]-methionine as well as the corresponding stoichiometries of the coatomer subunits are shown in Fig. A 3. Characterization of anti-coatomer antibody 883 is depicted in Fig. A 4. abstract_translated_lang: eng date: 2003 date_type: published id_scheme: DOI id_number: 10.11588/heidok.00004012 ppn_swb: 1643531395 own_urn: urn:nbn:de:bsz:16-heidok-40123 language: eng bibsort: WEGMANNDOMSUPPLEMENT2003 full_text_status: public citation: Wegmann, Dominik ; Hess, Pablo ; Baier, Carola ; Wieland, Felix T. ; Reinhard, Constanze (2003) Supplemental Materials: Novel Isotypic g/z-Subunits Reveal Three Coatomer Complexes in Mammals. [Other] document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/4012/1/Wegmann_et_al_Appendix_MCB.pdf