<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "STED-4Pi Microscopy"^^ . "Saturated stimulated emission depletion (STED) has been shown to break Abbe's resolution limit in fluorescence microscopy. This technique suppresses fluorescence at the focal rim by use of a depleting laser pulse. In this thesis, theoretical and experimental examinations show that the combination with another technique, namely 4Pi-confocal microscopy, leads to a yet unreached axial resolution in light microscopy. The central part of this work is the performance of a STED-4Pi experiment. Simulations predict a superresolution of about 30 nm that is confirmed in experiments with ultrathin fluorescent layers. These results are so far considered to have the highest axial resolution for a light microscope. First biological images are recorded with membrane labeled bacteria at 40-50 nm resolution. A major issue is the photostability of the fluorescent dyes. With the bacterial samples, it can be exemplarily demonstrated that longer depletion pulses increase photostability. An amino-reactive dye, that is stable under STED conditions, enabled the first superresolved mammalian immuno-fluorescence images. They reveal a resolution of 50 nm along the optic axis. Finally, a further implementation of an additional STED beam for lateral resolution enhancement is demonstrated. In future, this will enable simultaneous superresolution of at least 100 nm in the lateral and 50 nm in the axial direction."^^ . "2004" . . . . . . . . "Marcus"^^ . "Dyba"^^ . "Marcus Dyba"^^ . . . . . . "STED-4Pi Microscopy (PDF)"^^ . . . "Dissertation_Marcus_Dyba.pdf"^^ . . . "STED-4Pi Microscopy (Other)"^^ . . . . . . "small.jpg"^^ . . . "STED-4Pi Microscopy (Other)"^^ . . . . . . "medium.jpg"^^ . . . "STED-4Pi Microscopy (Other)"^^ . . . . . . "preview.jpg"^^ . . . "STED-4Pi Microscopy (Other)"^^ . . . . . . "lightbox.jpg"^^ . . "HTML Summary of #4438 \n\nSTED-4Pi Microscopy\n\n" . "text/html" . . . "530 Physik"@de . "530 Physics"@en . .