%0 Generic
%A Sawinski, Jürgen
%D 2005
%F heidok:6025
%K Faser-Scanner , Miniatur-MikroskopFiber-Scanne , Head-mount Microscope
%R 10.11588/heidok.00006025
%T Development of a head-mount fiber scanning system for imaging in vivo
%U https://archiv.ub.uni-heidelberg.de/volltextserver/6025/
%X In this thesis I will describe the basic concepts necessary for acquiring functional data from nerve tissue by application of two-photon laser scanning microscopy and its miniaturization for in vivo imaging in freely moving animals. The construction of a laser with enhanced repetition rate and the design of a novel non-resonant fiber scanning technique is emphasized. The increased repetition rate of the femtosecond laser source should decrease undesirable non-linear effects when transmitting ultra-short pulses with a single mode fiber. By implementing the prototype of a head-mounted microscope involving the novel fiber scanner and testing with fluorescent probes I can demonstrate its feasibility - not only for imaging in freely moving animals but for mobile laser scanning microscopy in general.