title: A new strategy for the treatment of viral infections
creator: Wolf, Markus
subject: 610
subject: 610 Medical sciences Medicine
description: Intact viruses, containing no viral genome but which are loaded with a therapeutic drug or virus like particles loaded with antiviral drugs are potentially selective drug carriers for the intracellular delivery of therapeutics. These virus constructs could be used as therapeutics for the therapy of viral infections. For example HI-viruses could be designed, that are loaded with antisense oligonucleotides that avoid replication of the wild type HI virus. The advantage of such antisense oligonucleotide loaded viruses could be the fact that they enter cells and deliver the therapeutics to the host cells of the wild type HI virus. It should be possible to treat all viral infections with this method. Wild type viruses are therapied by a modified virus of the same type or a virus like particle containing a antiviral drug – e.g. an antisense oligonucleotide. This method should work for all viruses. Additionally, a new method is described how intact viruses, containing no viral genome but which are loaded with a therapeutic drug could be obtained. The major obstacle is that the viral genome and viral proteins must be present for virus assembly on the one hand, on the other hand it must be removed subsequent to virus assembly in order to obtain a safe drug carrier. The idea is: Virus assembly takes place in the presence of the viral genome with subsequent removal of the viral genome. Firstly the viral genome is immobilized on a solid support – comparable to DNA chips. Virus assembly proceeds at the solid support. After virus assembly and drug loading the immobilized viral genome is removed. Viral proteins could be obtained from a packaging cell line. The lysate of such a packaging cell line containing the viral proteins is incubated with the immobilized viral genome to allow virus assembly. The lysate is removed after succesful assembly. The next step would be the loading of the virus with a therapeutic drug. As the viral proteins contain amino and carboxy groups they can be chemically modified by a therapeutic drug. Finally the drug loaded virus is removed from the solid support and with it from the viral genome. This could be a strategy to obtain drug loaded, intact viruses lacking the viral genome.
date: 2006
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/6592/1/markuswolf_virus_therapy.pdf
identifier: DOI:10.11588/heidok.00006592
identifier: urn:nbn:de:bsz:16-opus-65923
identifier:   Wolf, Markus  (2006) A new strategy for the treatment of viral infections.         
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/6592/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng