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primary topic: Design of Artificial Modular Extracellular Matrices

Design of Artificial Modular Extracellular Matrices
type: Article
abstract: Cellular functions such as cell growth, adhesion and differentiation are essentially controlled by the surrounding extracellular matrix (ECM). The mechanical, chemical and structural properties of the ECM are consequently crucial for the selection of cells at interfaces and the formation of tissues. The objective of this thesis was to develop an artificial ECM to determine and control the parameters influencing the crosstalk between cells and their surroundings on a molecular level. Artificial ECMs which mimic the natural environment of cells enable precise insights into cell-ECM crosstalk; ultimately, we aim to trigger the crosstalk, such that specific cell functions are provoked. To this end, a modular ECM system was developed, consisting of (i) poly(ethylene glycol) (PEG) as the basic material, (ii) gold nano-particles as the structuring component, and (iii) bioactive molecules which are immobilized on the basic material and on the nano-structure, to equip these modules with a biological function. The mechanical, structural, and chemical properties of the artificial ECM, as defined by the respective modules, can be tuned independently from one another, enabling the customized tailoring of the artificial ECM for specific applications. PEG hydrogels, used as both the basic material and first module of the artificial ECM, were chosen because of their resistance to protein adsorption, as well as their elastic and swelling properties, which partly mimic the hyaluronan material surrounding the cell membrane. A photo-initiated crosslinking reaction of PEG macromers was used to obtain hydrogels with well-controlled physical properties, characterized in terms of the gel content, swelling ratio, and mesh size. Elasticity at the nanoscale was assessed by an indentation method using atomic force microscopy (AFM). In this way, we were able to prepare hydrogel surfaces covering the biologically relevant range of elasticities. Structuring the hydrogel substrates with nanoscale …
date: 2006
status: published
author list: authors
type: EPrint
type: DoctoralThesisEPrint
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subject: 540 Chemie

Design of Artificial Modular Extracellular Matrices (PDF)
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Stefan V. W. Gräter
type: Person
given name: Stefan V. W.
family name: Gräter

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primary topic: Design of Artificial Modular Extracellular Matrices

540 Chemie
type: Concept
preferred label: 540 Chemistry and allied sciences @en