title: Modulation of GABAergic transmission in the cerebellar stellate cell network by neurotransmitter spillover and synaptic cross talk
creator: Astori, Simone
subject: 530
subject: 530 Physics
description: Cerebellar stellate cells are interconnected interneurons located in the outer molecular layer. They provide inhibition to Purkinje cells, thereby modulating the final output of the cerebellum. In this work, electrophysiological means were employed to study the stellate cell network at the single synapse level. Sustained excitatory input invading the molecular layer was found to depress the chemical transmission between stellate cells, due to a decrease in the release probability of the inhibitory neurotransmitter ?-Aminobutyric acid (GABA). Analysis of synaptic parameters combined with the application of pharmacological tools indicated the involvement of metabotropic GABAB receptors and ionotropic L-?-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-receptors localized in the axon of stellate cells. Ca2+ imaging experiments performed with Two-photon Microscopy confirmed the role of presynaptic receptors in affecting the axonal Ca2+ signals that trigger GABA release. These findings support the relevance of presynaptic receptors and provide another example of neurotransmitter spillover and cross talk between synapses, enriching the scenario of signal transmission at central synapses.
date: 2006
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/7032/1/thesis_astori.pdf
identifier: DOI:10.11588/heidok.00007032
identifier: urn:nbn:de:bsz:16-opus-70325
identifier:   Astori, Simone  (2006) Modulation of GABAergic transmission in the cerebellar stellate cell network by neurotransmitter spillover and synaptic cross talk.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/7032/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng