…
comment: The repository administrator has not yet configured an RDF license.
primary topic: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites

Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites
type: Article
abstract: Summary Leishmania HASPB is a lipoprotein that is exported to the extracellular space from both Leishmania parasites and mammalian cells via an unconventional secretory pathway (Denny et al., 2000; Stegmayer et al., 2005). Exported HASPB remains anchored in the outer leaflet of the plasma membrane mediated by myristate and palmitate residues covalently attached to the N-terminal SH4 domain of HASPB (Denny et al., 2000; Stegmayer et al., 2005). HASPB targeting to the plasma membrane depends on SH4 acylation, which occurs at intracellular membranes. How acylated HASPB is targeted to the plasma membrane and, in particular the subcellular site of HASPB membrane translocation is unknown. Furthermore, possible secretory mechanisms for HASPB are not yet clarified in molecular terms. In order to address this issue, a screening for clonal CHO mutants derived from somatic mutagenesis that are incapable of exporting HASPB, was performed. In such a CHO mutant cell line HASPB was myristoylated and palmitoylated and was mainly localized to the plasma membrane as judged by confocal microscopy and subcellular fractionation. However, based on a quantitative flow cytometry assay and a biochemical biotinylation assay of surface proteins, HASPB transport to the outer leaflet of the plasma membrane was largely reduced in this mutant despite a normal expression level of the HASPB reporter molecule compared to CHO wild-type cells. These findings indicate that the subcellular site of HASPB membrane translocation is the plasma membrane as the reporter molecule accumulates in this location when export is blocked. Hence, based on these results a two-step process of HASPB cell surface biogenesis can be defined, in which SH4 acylation of HASPB mediates intracellular targeting to the plasma membrane. In a second step, the plasma membrane-resident machinery that is apparently disrupted in the CHO mutant cell line, mediates membrane translocation of HASPB. Intriguingly, the angiogenic growth facto…
date: 2006
status: published
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
has document: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (Other)
subject: 570 Biowissenschaften, Biologie

Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
type: Document
has file: stegmayer_phd.pdf
has part: stegmayer_phd.pdf

Carolin Stegmayer
type: Person
given name: Carolin
family name: Stegmayer

Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (Other)
type: Document
is version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
is volatile version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
is index codes version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
has file: indexcodes.txt
has part: indexcodes.txt

Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (Other)
type: Document
is version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
is volatile version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
islightbox thumbnail version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
has file: lightbox.jpg
has part: lightbox.jpg

Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (Other)
type: Document
is version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
is volatile version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
ispreview thumbnail version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
has file: preview.jpg
has part: preview.jpg

Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (Other)
type: Document
is version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
is volatile version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
ismedium thumbnail version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
has file: medium.jpg
has part: medium.jpg

Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (Other)
type: Document
is version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
is volatile version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
issmall thumbnail version of: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites (PDF)
has file: small.jpg
has part: small.jpg

HTML Summary of #7041 Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites
format: text/html
primary topic: Molecular Analysis of the Unconventional Export Machinery of HASPB, a component of the surface coat of Leishmania parasites

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en