title: Forces Are Able to Set Position And Timing Of Microtubule Catastrophes In Fission Yeast
creator: Foethke, Dietrich
subject: ddc-570
subject: 570 Life sciences
description: In the fission yeast Schizosaccharomyces pombe, interphase microtubules switch to depolymerization almost exclusively at the poles of the cylindrical cell. Proper localization of these events is crucial for morphogenesis, and is thought to depend on factors preferentially localized at the cell poles. Using computer simulations we analyzed five different models of how microtubule dynamic instability might be regulated in order to reproduce the organization of microtubules observed in vivo. To evaluate the simulations we compared their output to nine traits of interphase cells. Using the simulation we could show that the shape of fission yeast induces forces on microtubule plus ends specifically at the cell poles. We found that the effect of these forces on microtubule dynamics was sufficient to reproduce the nine traits without the requirement of pole-specific factors. In vivo experiments with mutant cells confirmed that cell shape is essential for the proper organization of microtubules in fission yeast which indicates that force might also be relevant in living cells. We furthermore applied the simulation to study the organization of microtubules in mutant cells that were deleted of the microtubule tip tracking proteins Mal3 and Tip1. This lead to the proposal of a novel hypothesis how Tip1 might function in vivo to assist force in the discrimination of the cell poles from central parts of the cell cortex.
date: 2007
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/7312/1/phd_thesis_dietrich_foethke_300dpi.pdf
identifier: DOI:10.11588/heidok.00007312
identifier: urn:nbn:de:bsz:16-opus-73129
identifier:   Foethke, Dietrich  (2007) Forces Are Able to Set Position And Timing Of Microtubule Catastrophes In Fission Yeast.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/7312/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng