<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Epithelial and cellular innate immune responses of Anopheline mosquitoes to Plasmodium parasites"^^ . "Anopheles gambiae is the most prominent vector of human malaria in Africa. The causative agents of this disastrous disease are unicellular eukaryotic parasites of the genus Plasmodium which are transmitted to humans by infected female mosquitoes when they take a blood meal. During its development in the mosquito, Plasmodium undergoes massive losses suggesting that mosquitoes are able to mount an immune response that limits parasite infection. However, the molecular mechanisms underlying parasite invasion, immune evasion, its recognition and killing via the vector are not well understood. We have functionally analysed the orthologous SRPN6 genes from Anopheles stephensi and Anopheles gambiae and showed that they are specifically expressed in midgut cells invaded by Plasmodium ookinetes. Phenotypic analysis via RNAi knock down indicates that AsSRPN6 is involved in the parasite killing process, whereas AgSRPN6 acts on parasite clearance, by promoting parasite lysis. Furthermore, SRPN6 is also a parasite induced salivary gland epithelial marker located at the basal side of epithelial cells in proximity to invading sporozoites. Knockdown of SRPN6 had no effect on oocyst rupture but significantly increased the number of sporozoites present in salivary glands. Midgut invasion is vital for the parasite life cycle progression. We show that Δpplp5 ookinetes cannot invade midgut epithelial cells and are retained attached to the midgut, possibly, due to the fact that, these mutant parasites can not form pores in the plasma membrane. Haemocytes secrete immune factors such as opsonins, proteases and their negative regulators as well as antimicrobial peptides, all crucial for immune responses. We report the first genome wide molecular characterisation of Anopheles gambiae circulating haemocytes, presenting a list of 1587 genes we strongly suggest are expressed by these cells. "^^ . "2007" . . . . . . . . "Sofia B."^^ . "Pinto"^^ . "Sofia B. Pinto"^^ . . . . . . "Epithelial and cellular innate immune responses of Anopheline mosquitoes to Plasmodium parasites (PDF)"^^ . . . "Thesis_SBPinto.pdf"^^ . . . "Epithelial and cellular innate immune responses of Anopheline mosquitoes to Plasmodium parasites (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Epithelial and cellular innate immune responses of Anopheline mosquitoes to Plasmodium parasites (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Epithelial and cellular innate immune responses of Anopheline mosquitoes to Plasmodium parasites (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Epithelial and cellular innate immune responses of Anopheline mosquitoes to Plasmodium parasites (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Epithelial and cellular innate immune responses of Anopheline mosquitoes to Plasmodium parasites (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #7355 \n\nEpithelial and cellular innate immune responses of Anopheline mosquitoes to Plasmodium parasites\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .