<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Analysis of the role of estrogen receptor alpha in cerebral stroke"^^ . "The hormone 17beta-estradiol (E2) and its receptor estrogen receptor alpha (ERalpha) have neuroprotective effects in animal models of stroke in rodents. In contrast, clinical studies revealed, that long term treatment with estrogens lead to an increased risk of dementia and stroke. These controversy shows, that there is a need for a better understanding of E2 and ERalpha action in stroke. To investigate the role of ERalpha in stroke, three cell type specific ERalpha knock out mouse-strains were generated using the Cre-loxP system: a neuronal specific ERalpha knock out mouse strain (CaMKIICre/ERfl/fl), a microglial specific ERalpha knock out mouse strain (LysMCre/ERfl/fl) and an endothelial specific ERalpha knock mouse strain (Tie2CreERT2/ERfl/fl). These mouse-strains were analysed for tissue specific deletion of ERalpha. Deletion of ERalpha in neurons of CaMKIICre/ERfl/fl-mice was complete, in LysMCre/ERfl/fl-mice ERalpha was deleted in 92% of the microglial cells whereas the deletion of ERalpha was incomplete in endothelial cells of the vascular system in the Tie2CreERT2/ERfl/fl-mice. Due to these results the Tie2CreERT2/ERfl/fl mouse-strain was excluded for further experiments. To investigate the role of ERalpha in neurons and in microglial cells in stroke, experiments using a model of middle cerebral artery occlusion (MCAO) were performed. Analysing the stroke volume after performing a MCAO in CaMKIICre/ERfl/fl-mice and in LysMCre/ERfl/fl-mice revealed, that it is neuronal ERalpha and not microglial ERalpha which mediates the neuroprotective effects of E2 in stroke. Furthermore it was shown, that E2 has neuroprotective effects in female as well as in male mice, and that in both sexes the neuroprotecive effect of E2 is mediated via neuronal ERalpha. For a better understanding of the molecular mechanisms underlying these neuroprotective effects mediated by neuronal ERalpha, the expression of several genes in female CaMKIICre/ERfl/fl-mice was investigated. It was shown in this work, that ERalpha is upregulated in stroke, whereas Bcl-2, Summary 4 cocaine- and amphetamine-regulated transcript (CART), cyclooxygenase 2 (COX-2), prostaglandin E2 EP1 receptor (EP1) and prostaglandin E2 EP2 receptor (EP2) transcription was unchanged. Brain derived neurotrophic factor (BDNF) was upregulated upon E2 treatment in stroke. The upregulation of BDNF was independent from neuronal ERalpha since its transcription was elevated in the neuronal ERalpha knock out mice as well. Taken together, it was demonstrated in this work, that neuronal ERalpha and not microglial ERalpha plays a major role in E2 mediated neuroprotection in stroke."^^ . "2007" . . . . . . . . "Joachim"^^ . "Elzer"^^ . "Joachim Elzer"^^ . . . . . . "Analysis of the role of estrogen receptor alpha in cerebral stroke (PDF)"^^ . . . "Hey_Ho_final_richtig.pdf"^^ . . . "Analysis of the role of estrogen receptor alpha in cerebral stroke (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Analysis of the role of estrogen receptor alpha in cerebral stroke (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Analysis of the role of estrogen receptor alpha in cerebral stroke (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Analysis of the role of estrogen receptor alpha in cerebral stroke (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Analysis of the role of estrogen receptor alpha in cerebral stroke (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #7376 \n\nAnalysis of the role of estrogen receptor alpha in cerebral stroke\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .