TY  - GEN
ID  - heidok7405
Y1  - 2007///
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/7405/
KW  - Nanoskopie 
KW  -  Beugungsunbegrenzt 
KW  -  RESOLFTnanoscopy 
KW  -  diffraction-unlimited 
KW  -  STED 
KW  -  RESOLFT
AV  - public
TI  - STED microscopy with Q-switched microchip lasers
A1  - Kellner, Robert Rudolf
N2  - The far-field light microscope is a valuable scientific instrument in modern life sciences, nevertheless, its full potential was constrained by the diffraction-limited resolution. A concept exploiting a saturable optical transition of fluorescent molecules, namely stimulated emission depletion (STED), allowed to overcome this restriction and provides diffraction-unlimited resolution in far-field light microscopy. In this thesis STED microscopy as a tool in cell biology is further established by revealing the ring-like structure of the Bruchpilot protein in larval Drosophila neuromuscular junctions and clustering behavior of the nicotinic acetylcholine receptor in mammalian cells. Moreover, the performance of STED microscopes is further improved by examining photobleaching behavior of fluorescent dyes and presenting a new illumination scheme for reduced photobleaching and increased signal. For the first time a new approach to STED microscopy with a Q-switched microchip laser, replacing a Ti:Sapphire-based laser system, was successfully implemented. The applicability of this approach and an increase in resolution beyond the diffraction limit to below 25 nm is demonstrated with colloidal nanoparticles and in biological samples. The range of applicable dyes for STED microscopy is further extended into the blue regime, widening the field of possible applications.
ER  -