<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Tumour selective replication of Newcastle Disease Virus as the result of defects in the basal and inducible expression of interferon-related proteins"^^ . "Newcastle Disease Virus (NDV) is an avian Paramyxovirus that preferentially replicates in cancer cells and that has already been used successfully in the treatment of cancer. It has been suggested that defects in the interferon response present in many neoplastic cells make them prone to NDV infection. However, the exact mechanisms that underlie the tumour selective replication of NDV are not known. In this thesis the relation between the NDV susceptibility of cells and the basal and inducible expression of different type I interferon-related proteins was analysed. It was assessed what the differences in the interferon response were between tumour and normal cells and if these differences could explain the tumour selectivity of NDV. It could be shown that tumour cells have defects in the basal and inducible expression of several antiviral, interferon-related genes. These genes include cytosolic viral RNA receptors such as RIG-I, transcription factors such as IRF3 and IRF7, type I interferons and antiviral effector molecules such as PKR. In addition tumour cells displayed defects in the responsiveness to interferon pretreatment with regard to the induction of an antiviral state by the upregulation of antiviral genes. A statistically significant, negative correlation was found between the expression of many of the tested interferon-related genes and the susceptibility to NDV infection. Furthermore the functional significance of some of the genes for the establishment of an antiviral response could be evaluated by the analysis of gene knock out macrophages. These functional analyses revealed the crucial importance of the type I interferon receptor and to a lesser degree also of IRF3/7 for NDV resistance and interferon-related gene expression. Some tumour cells were found to be unusually resistant to NDV infection, which could be connected to an exceptionally strong expression of antiviral genes. All in all the results strongly indicate multiple defects in the interferon response of tumour cells as the cause for the tumour selective replication of NDV. There seems to be a general downregulation of the antiviral gene expression rather than defects in the expression of only a few interferon-related genes. These findings shed new light on the processes that take place in normal and tumour cells after NDV infection. In the future they might be used to improve the efficacy of cancer treatment with NDV by the prediction of the virus susceptibility of a tumour with the help of gene expression analysis before virus treatment."^^ . "2007" . . . . . . . . "Holger"^^ . "Wilden"^^ . "Holger Wilden"^^ . . . . . . "Tumour selective replication of Newcastle Disease Virus as the result of defects in the basal and inducible expression of interferon-related proteins (PDF)"^^ . . . "PhD_thesis_Holger_Wilden.pdf"^^ . . . "Tumour selective replication of Newcastle Disease Virus as the result of defects in the basal and inducible expression of interferon-related proteins (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Tumour selective replication of Newcastle Disease Virus as the result of defects in the basal and inducible expression of interferon-related proteins (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Tumour selective replication of Newcastle Disease Virus as the result of defects in the basal and inducible expression of interferon-related proteins (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Tumour selective replication of Newcastle Disease Virus as the result of defects in the basal and inducible expression of interferon-related proteins (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Tumour selective replication of Newcastle Disease Virus as the result of defects in the basal and inducible expression of interferon-related proteins (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #7483 \n\nTumour selective replication of Newcastle Disease Virus as the result of defects in the basal and inducible expression of interferon-related proteins\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .