title: Ensconsin, a Par-1 regulated microtubule associated protien, regulates kinesin dependent transport
creator: Sung, Hsin-Ho
subject: 570
subject: 570 Life sciences
description: Maternal proteins and mRNA contribute to both oocyte and embryo development. I performed a genetic screen to identify genes with maternal function in Drosophila. From this screen, I isolated PB4170, which affects CG14998 (ensconsin). The human homologue of this protein, E-MAP-115/Ensconsin, is known to be a microtubule binding protein that interacts dynamically with microtubules. However, its molecular function is poorly understood. From ensconsin mutant analysis in Drosophila, I have found that this gene is specifically required for microtubule-dependent polarity in the oocyte. My results suggest that Ensconsin does not directly affect the stability or orientation of microtubules, but acts through Kinesin, a plus end directed motor that moves cargos along microtubules. I could also demonstrate that Ensconsin is a target of the Par-1 kinase, which has been shown to be required for the establishment of oocyte polarity in Drosophila. In mammals, the Par-1 homolog MARK destabilises microtubules through phosphorylation of microtubule associated proteins (MAPs).In Drosophila, Par-1 directly affects microtubule stability via unkown MAPs, as the MAPs identified so far do not show any polarity defects in the oocyte. As ensconsin mutants do show an oocyte polarity phenotype, this strongly suggests that in Drosophila Par-1 kinase controls oocyte polarity also through the regulation of Kinesin-based transport by phosphorylation of Ensconsin.
date: 2007
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/7662/1/thesis_4pdf.pdf
identifier: DOI:10.11588/heidok.00007662
identifier: urn:nbn:de:bsz:16-opus-76624
identifier:   Sung, Hsin-Ho  (2007) Ensconsin, a Par-1 regulated microtubule associated protien, regulates kinesin dependent transport.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/7662/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng