…
comment: The repository administrator has not yet configured an RDF license.
primary topic: Parallelized Ground State Depletion

Parallelized Ground State Depletion
type: Article
abstract: RESOLFT was introduced to break the limited resolution in fluorescence microscopy given by thephysical limit of diffraction. RESOLFT needs saturable fluorescence depletion and illumination with spatial regions of zero intensity. Improvements in RESOLFT are still essential since (i) photobleaching restricts the use of large intensities obligate for high resolution, (ii) long acquisition times emerge from single-point scanning, and (iii) RESOLFT puts ahead special requirements on the dye´s photophysical properties. To counteract these problems, this work presents a RESOLFT-type microscope implementing parallelized ground state depletion (GSD). Parallelization allows rather fast image acquisition applying structured illumination combined with widefield detection on a camera. The depletion of the fluophore´s ground state, and thus the fluorescence, is realized using a pump-probe illumination scheme. To increase photostability, the sample was evacuated and cooled to approximately 80 K. Since the population of the triplet state is an intrinsic property of almost every dye and is enhanced by evacuation and low temperatures, parallelized GSD is generally applicable to a wide range of fluorescent markers. Applying parallelized RESOLFT an enhancement in resolution was proven for a bead sample as well as inside a cell. Parallelized GSD microscopy further prevents photostress on both fluorescent label and sample, since it encounters moderate illumination intensities < kW/cm2.
date: 2007
status: published
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Parallelized Ground State Depletion (PDF)
has document: Parallelized Ground State Depletion (Other)
subject: 530 Physik

Parallelized Ground State Depletion (PDF)
type: Document
has file: Miriam_Schwentker_diss.pdf
has part: Miriam_Schwentker_diss.pdf

Miriam A. Schwentker
type: Person
given name: Miriam A.
family name: Schwentker

Parallelized Ground State Depletion (Other)
type: Document
is version of: Parallelized Ground State Depletion (PDF)
is volatile version of: Parallelized Ground State Depletion (PDF)
is index codes version of: Parallelized Ground State Depletion (PDF)
has file: indexcodes.txt
has part: indexcodes.txt

Parallelized Ground State Depletion (Other)
type: Document
is version of: Parallelized Ground State Depletion (PDF)
is volatile version of: Parallelized Ground State Depletion (PDF)
islightbox thumbnail version of: Parallelized Ground State Depletion (PDF)
has file: lightbox.jpg
has part: lightbox.jpg

Parallelized Ground State Depletion (Other)
type: Document
is version of: Parallelized Ground State Depletion (PDF)
is volatile version of: Parallelized Ground State Depletion (PDF)
ispreview thumbnail version of: Parallelized Ground State Depletion (PDF)
has file: preview.jpg
has part: preview.jpg

Parallelized Ground State Depletion (Other)
type: Document
is version of: Parallelized Ground State Depletion (PDF)
is volatile version of: Parallelized Ground State Depletion (PDF)
ismedium thumbnail version of: Parallelized Ground State Depletion (PDF)
has file: medium.jpg
has part: medium.jpg

Parallelized Ground State Depletion (Other)
type: Document
is version of: Parallelized Ground State Depletion (PDF)
is volatile version of: Parallelized Ground State Depletion (PDF)
issmall thumbnail version of: Parallelized Ground State Depletion (PDF)
has file: small.jpg
has part: small.jpg

HTML Summary of #7677 Parallelized Ground State Depletion
format: text/html
primary topic: Parallelized Ground State Depletion

530 Physik
type: Concept
preferred label: 530 Physics @en