<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Functional characterization of Lupli, a new component of the oskar RNP complex in the Drosophila oocyte"^^ . "mRNA localization is a common mechanism for targeting proteins to specific locations within a cell, and is crucial for establishment of cellular asymmetries. In Drosophila, the two main body axis are determined by the localization of three maternal transcripts in the oocyte, bicoid, gurken, and oskar mRNA. oskar mRNA localizes to the posterior pole of the oocyte where it is translated locally, which is crucial for abdomen formation and germline development. Many of the factors regulating this process have been identified by means of genetics, and the majority of them colocalize with the oskar transcript at the posterior pole. However, the precise mechanism of oskar mRNA localization is still not understood. To gain further insight into the process of oskar regulation, I aimed at identifying novel components involved in this process. I participated in a protein-trap screen where GFP fusions were randomly generated, and colocalization with oskar mRNA at the posterior pole of the oocyte was scored. Several lines displaying an enriched GFP signal at the posterior pole were identified. I characterized an independently generated line, in which the gene identified encodes a novel RNA recognition motif (RRM)-containing protein that I named Lupli. By immunostaining, I showed that endogenous Lupli protein displays the same distribution pattern in the oocyte as GFP::Lupli. Lupli associates with the 3’ UTR of oskar mRNA and is a component of the oskar ribonucleoprotein (RNP) complex in vivo. I showed that the late short-range transport of oskar mRNA to the cortex is defective in lupli mutant oocytes at stage 9, and that the microtubule cytoskeleton is not properly polarized. Furthermore, Oskar protein levels are reduced in lupli mutant ovaries. My work thus led to the identification of Lupli as a new component of the oskar RNP complex, and suggests that its function is required for proper localization and expression of oskar in Drosophila oocytes."^^ . "2007" . . . . . . . . "Lukas"^^ . "Neidhart"^^ . "Lukas Neidhart"^^ . . . . . . "Functional characterization of Lupli, a new component of the oskar RNP complex in the Drosophila oocyte (PDF)"^^ . . . "thesisLNeidhart.pdf"^^ . . . "Functional characterization of Lupli, a new component of the oskar RNP complex in the Drosophila oocyte (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Functional characterization of Lupli, a new component of the oskar RNP complex in the Drosophila oocyte (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Functional characterization of Lupli, a new component of the oskar RNP complex in the Drosophila oocyte (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Functional characterization of Lupli, a new component of the oskar RNP complex in the Drosophila oocyte (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Functional characterization of Lupli, a new component of the oskar RNP complex in the Drosophila oocyte (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #7752 \n\nFunctional characterization of Lupli, a new component of the oskar RNP complex in the Drosophila oocyte\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .