<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Generation and analysis of transgenic mice expressing CRE recombinase in defined interneurons"^^ . "GABAergic interneurons are the main source of inhibition in the central nervous system. In addition they play a crucial role during development since in a paradoxical fashion they are the origin of the first excitatory signals in the immature brain. GABAergic interneurons comprise about 10 – 20% of the neuronal cell population and they can be divided into several subtypes. GABAergic interneuron classifications have been based on different criteria, including anatomical, neurochemical or physiological characteristics. Although the overall number of interneurons is small compared to that of principal cells, by virtue of their connectivity, interneurons are able to shape and regulate the activity of numerous principal cells and thus influence network activity. To elucidate the role of interneurons during development and in the mature brain specific modifications of their molecular and physiological properties are required and can be achieved by selective ablation of distinct genes. An established and widely used technique is that of the CRE/loxP system. For ablation of a desired gene in a cell-type specific fashion the generation of mice expressing CRE recombinase in a subset of cells plus the generation of mice with a floxed allele are a prerequisite. The aim of this study was the generation of mice with CRE expression in all GABAergic interneurons and of mice with restricted expression of CRE recombinase in a subset of GABAergic interneurons, the somatostatin-positive interneurons. To this end mice with CRE expression under the control of the GAD67 promoter (GAD67CRE/+) - a common feature of almost all interneurons - and mice with CRE recombinase expression under the control of the somatostatin promoter (SOMCRE/+) were generated. Immunohistochemical analysis of both GAD67CRE/+ and SOMCRE/+ mice provided evidence that CRE recombinase is functional in vivo. Co-localisation studies of CRE recombinase and endogenous GAD67 expression, demonstrated a 100% overlap. Double-labelling experiments of endogenous somatostatin and CRE recombinase demonstrated a good correspondence in the hippocampus but less so in other brain regions. No developmental or behavioural deficits were observed as a consequence of the genetic manipulations. Cell-type specific ablations of several genes of interest, e.g. trkB receptors in GABAergic interneurons, NR1 and GluR-A subunits in somatostatin-positive interneurons are currently being generated and will help provide more insights into the function of GABAergic interneurons during development and their involvement in specific network activity. "^^ . "2007" . . . . . . . . "Angelika"^^ . "Vogt"^^ . "Angelika Vogt"^^ . . . . . . "Generation and analysis of transgenic mice expressing CRE recombinase in defined interneurons (PDF)"^^ . . . "Thesis_AV.pdf"^^ . . . "Generation and analysis of transgenic mice expressing CRE recombinase in defined interneurons (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Generation and analysis of transgenic mice expressing CRE recombinase in defined interneurons (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Generation and analysis of transgenic mice expressing CRE recombinase in defined interneurons (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Generation and analysis of transgenic mice expressing CRE recombinase in defined interneurons (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Generation and analysis of transgenic mice expressing CRE recombinase in defined interneurons (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #7926 \n\nGeneration and analysis of transgenic mice expressing CRE recombinase in defined interneurons\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .