title: Regulation and targets of Mal-D during border cell migration in Drosophila melanogaster oogenesis 
creator: Kanca, Oguz
subject: ddc-570
subject: 570 Life sciences
description: Border cells, a group of specialized follicle cells that commit collective migration during the oogenesis of Drosophila, constitute a useful migration model. Previous work in our laboratory by Kalman Somogyi identified Mal-D, a transcriptional co-activator of DSRF, is important for border cell migration. mal-D mutation causes decrease of F-Actin levels and loss of cellular integrity in border cells. Moreover Mal-D was found to accumulate in the nucleus of some border cells while the cluster is migrating and only if the cluster is migrating. A suggested mechanism was that the border cells receive a migration related signal, such as an increase of cellular tension and send Mal-D to the nucleus. The first part of my project was to understand how Mal-D is regulated by the migration. In order to visualize subcellular distribution of Mal-D I generated a tagged version of the endogenous protein by using homologous recombination. Analysis of subcellular distribution of Mal-D with this tool showed that the increase in nuclear levels of Mal-D in migrating cells is the result of an overall increase in the level of Mal-D protein and not redistribution of a fixed amount of protein. Furthermore I identified that mutations in Profilin or DSRF affect the nuclear levels of Mal-D. In the second part of my project I focused on the targets of Mal-D. I isolated border cell mutant for Mal-D or wild-type, and I compared their gene expression profiles by using microarrays.
date: 2007
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/7953/1/thesis_Oguz_Kanca.pdf
identifier: DOI:10.11588/heidok.00007953
identifier: urn:nbn:de:bsz:16-opus-79535
identifier:   Kanca, Oguz  (2007) Regulation and targets of Mal-D during border cell migration in Drosophila melanogaster oogenesis.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/7953/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng