<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA"^^ . "E. coli P48 protein is homologous to the SRP54 component of the eukaryotic signal recognition particle. In vivo, P48 is associated with 4.5S RNA which shares a homology with eukaryotic SRP RNA. To study the interaction between P48 and 4.5S RNA in vitro, we used 4.5S RNA with fluorescein coupled to the 3'-terminal ribose. Upon binding of P48, the fluorescent 4.5S RNA shows a substantial decrease in fluorescence. Fluorescence quenching as well as anisotropy measurements reveal that the effect is not due to a direct interaction of P48 with the dye. This suggests that the binding of P48 induces a conformational change in 4.5S RNA which affects the structure at the 3' end of the RNA. From equilibrium titrations with fluorescent 4.5S RNA, a dissociation constant of 0.15 microns is obtained for the RNA.protein complex. The formation of the complex is not affected by GTP binding to or hydrolysis by P48."^^ . "1994" . . . "348" . . "FEBS Letters"^^ . . . . . . . . . . . . . "Wolfgang"^^ . "Wintermeyer"^^ . "Wolfgang Wintermeyer"^^ . . "Bernhard"^^ . "Dobberstein"^^ . "Bernhard Dobberstein"^^ . . "Georg"^^ . "Lentzen"^^ . "Georg Lentzen"^^ . . . . . . "Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA (PDF)"^^ . . . "Lentzen_1994_FEBSLett.pdf"^^ . . . "Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #8302 \n\nFormation of SRP-like particle induces a conformational change in E. coli 4.5S RNA\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .