title: An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY
creator: Joen, Luirink
creator: ten Hagen-Jongman, Corinne M.
creator: van der Weijden, Coen C.
creator: Oudega, Bauke
creator: High, Stephen
creator: Dobberstein, Bernhard
creator: Kusters, Ron
subject: 570
subject: 570 Life sciences
description: In Escherichia coli, a signal recognition particle (SRP) has been identified which binds specifically to the signal sequence of presecretory proteins and which appears to be essential for efficient translocation of a subset of proteins. In this study we have investigated the function of E. coli FtsY which shares sequence similarity with the alpha-subunit of the eukaryotic SRP receptor ('docking protein') in the membrane of the endoplasmic reticulum. A strain was constructed which allows the conditional expression of FtsY. Depletion of FtsY is shown to cause the accumulation of the precursor form of beta-lactamase, OmpF and ribose binding protein in vivo, whereas the processing of various other presecretory proteins is unaffected. Furthermore, FtsY-depleted inverted cytoplasmic membrane vesicles are shown to be defective in the translocation of pre-beta-lactamase using an in vitro import assay. Subcellular localization studies revealed that FtsY is located in part at the cytoplasmic membrane with which it seems peripherally associated. These observations suggest that FtsY is the functional E. coli homolog of the mammalian SRP receptor.
date: 1994
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8335/1/Luirink_1994_EMBOJ.pdf
identifier: DOI:10.11588/heidok.00008335
identifier: urn:nbn:de:bsz:16-opus-83352
identifier:   Joen, Luirink ; ten Hagen-Jongman, Corinne M. ; van der Weijden, Coen C. ; Oudega, Bauke ; High, Stephen ; Dobberstein, Bernhard ; Kusters, Ron  (1994) An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY.  The EMBO Journal, 13 (10).  pp. 2289-2296.      
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8335/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng