eprintid: 8335
rev_number: 20
eprint_status: archive
userid: 1
dir: disk0/00/00/83/35
datestamp: 2008-04-10 11:34:31
lastmod: 2016-01-18 00:44:48
status_changed: 2012-08-14 15:25:08
type: article
metadata_visibility: show
creators_name: Joen, Luirink
creators_name: ten Hagen-Jongman, Corinne M.
creators_name: van der Weijden, Coen C.
creators_name: Oudega, Bauke
creators_name: High, Stephen
creators_name: Dobberstein, Bernhard
creators_name: Kusters, Ron
title: An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY
ispublished: pub
subjects: 570
divisions: 706000
abstract: In Escherichia coli, a signal recognition particle (SRP) has been identified which binds specifically to the signal sequence of presecretory proteins and which appears to be essential for efficient translocation of a subset of proteins. In this study we have investigated the function of E. coli FtsY which shares sequence similarity with the alpha-subunit of the eukaryotic SRP receptor ('docking protein') in the membrane of the endoplasmic reticulum. A strain was constructed which allows the conditional expression of FtsY. Depletion of FtsY is shown to cause the accumulation of the precursor form of beta-lactamase, OmpF and ribose binding protein in vivo, whereas the processing of various other presecretory proteins is unaffected. Furthermore, FtsY-depleted inverted cytoplasmic membrane vesicles are shown to be defective in the translocation of pre-beta-lactamase using an in vitro import assay. Subcellular localization studies revealed that FtsY is located in part at the cytoplasmic membrane with which it seems peripherally associated. These observations suggest that FtsY is the functional E. coli homolog of the mammalian SRP receptor.
abstract_translated_lang: eng
date: 1994
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008335
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 073
ppn_swb: 1349109223
own_urn: urn:nbn:de:bsz:16-opus-83352
language: eng
bibsort: JOENLUIRINANALTERNAT1994
full_text_status: public
publication: The EMBO Journal
volume: 13
number: 10
pagerange: 2289-2296
citation:   Joen, Luirink ; ten Hagen-Jongman, Corinne M. ; van der Weijden, Coen C. ; Oudega, Bauke ; High, Stephen ; Dobberstein, Bernhard ; Kusters, Ron  (1994) An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY.  The EMBO Journal, 13 (10).  pp. 2289-2296.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8335/1/Luirink_1994_EMBOJ.pdf