title: In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii
creator: Dobberstein, Bernhard
creator: Blobel, Günter
creator: Chua, Nam-Hai
subject: ddc-570
subject: 570 Life sciences
description: Translation of polyadenylated mRNA of Chlamydomonas reinhardtii in a cell-free wheat germ system resulted in the synthesis of numerous discrete polypeptides. Among them was a species with molecular weight 20,000 that was immunoprecipitated specifically by antibodies raised against the authentic small subunit (16,500 daltons) of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase(dimerizing), EC 4.1.1.39]. Since the immunoprecipitated polypeptide has a larger molecular weight by approximately 3500 than the small subunit (S) it was identified as a putative biosynthetic precursor (pS). Post-translational conversion of pS by a specific endoprotease yielded two detectable products: one apparently identical in size to S and the other, a small peptide, presumably representing the remainder of pS. The endoprotease requires sulfhydryl groups for its activity and is present in a C. reinhardtii postribosomal supernatant as well as in a free polysome fraction. The latter could account for the observation that completion of nascent chains in free polysomes yielded S but not pS. We propose that pS is an extacloroplastic form of S and that the smallfpeptide portion plays a role in the transfer of S into the chloroplast.
date: 1977
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8345/1/Dobberstein_1977_PNAS.pdf
identifier: DOI:10.11588/heidok.00008345
identifier: urn:nbn:de:bsz:16-opus-83459
identifier:   Dobberstein, Bernhard ; Blobel, Günter ; Chua, Nam-Hai  (1977) In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii.  Proc. Natl. Acad. Sci. USA, 74 (3).  pp. 1082-1085.      
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8345/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: ger