<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii"^^ . "Translation of polyadenylated mRNA of Chlamydomonas reinhardtii in a cell-free wheat germ system resulted in the synthesis of numerous discrete polypeptides. Among them was a species with molecular weight 20,000 that was immunoprecipitated specifically by antibodies raised against the authentic small subunit (16,500 daltons) of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase(dimerizing), EC 4.1.1.39]. Since the immunoprecipitated polypeptide has a larger molecular weight by approximately 3500 than the small subunit (S) it was identified as a putative biosynthetic precursor (pS). Post-translational conversion of pS by a specific endoprotease yielded two detectable products: one apparently identical in size to S and the other, a small peptide, presumably representing the remainder of pS. The endoprotease requires sulfhydryl groups for its activity and is present in a C. reinhardtii postribosomal supernatant as well as in a free polysome fraction. The latter could account for the observation that completion of nascent chains in free polysomes yielded S but not pS. We propose that pS is an extacloroplastic form of S and that the smallfpeptide portion plays a role in the transfer of S into the chloroplast."^^ . "1977" . . . "74" . "3" . . "Proc. Natl. Acad. Sci. USA"^^ . . . . . . . . . . . . . "Bernhard"^^ . "Dobberstein"^^ . "Bernhard Dobberstein"^^ . . "Nam-Hai"^^ . "Chua"^^ . "Nam-Hai Chua"^^ . . "Günter"^^ . "Blobel"^^ . "Günter Blobel"^^ . . . . . . "In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii (PDF)"^^ . . . "Dobberstein_1977_PNAS.pdf"^^ . . . "In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii (Other)"^^ . . . . . . "preview.jpg"^^ . . . "In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii (Other)"^^ . . . . . . "medium.jpg"^^ . . . "In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #8345 \n\nIn vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .