TY  - JOUR
JF  - Proc. Natl. Acad. Sci. USA
TI  - In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii
AV  - public
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/8345/
ID  - heidok8345
Y1  - 1977///
N2  - Translation of polyadenylated mRNA of Chlamydomonas reinhardtii in a cell-free wheat germ system resulted in the synthesis of numerous discrete polypeptides. Among them was a species with molecular weight 20,000 that was immunoprecipitated specifically by antibodies raised against the authentic small subunit (16,500 daltons) of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase(dimerizing), EC 4.1.1.39]. Since the immunoprecipitated polypeptide has a larger molecular weight by approximately 3500 than the small subunit (S) it was identified as a putative biosynthetic precursor (pS). Post-translational conversion of pS by a specific endoprotease yielded two detectable products: one apparently identical in size to S and the other, a small peptide, presumably representing the remainder of pS. The endoprotease requires sulfhydryl groups for its activity and is present in a C. reinhardtii postribosomal supernatant as well as in a free polysome fraction. The latter could account for the observation that completion of nascent chains in free polysomes yielded S but not pS. We propose that pS is an extacloroplastic form of S and that the smallfpeptide portion plays a role in the transfer of S into the chloroplast.
EP  - 1085
SP  - 1082
IS  - 3
A1  - Dobberstein, Bernhard
A1  - Blobel, Günter
A1  - Chua, Nam-Hai
VL  - 74
ER  -