eprintid: 8345
rev_number: 8
eprint_status: archive
userid: 1
dir: disk0/00/00/83/45
datestamp: 2008-04-10 12:44:17
lastmod: 2014-09-18 13:04:28
status_changed: 2012-08-14 15:25:08
type: article
metadata_visibility: show
creators_name: Dobberstein, Bernhard
creators_name: Blobel, Günter
creators_name: Chua, Nam-Hai
title: In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii
ispublished: pub
subjects: ddc-570
divisions: i-706000
abstract: Translation of polyadenylated mRNA of Chlamydomonas reinhardtii in a cell-free wheat germ system resulted in the synthesis of numerous discrete polypeptides. Among them was a species with molecular weight 20,000 that was immunoprecipitated specifically by antibodies raised against the authentic small subunit (16,500 daltons) of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase(dimerizing), EC 4.1.1.39]. Since the immunoprecipitated polypeptide has a larger molecular weight by approximately 3500 than the small subunit (S) it was identified as a putative biosynthetic precursor (pS). Post-translational conversion of pS by a specific endoprotease yielded two detectable products: one apparently identical in size to S and the other, a small peptide, presumably representing the remainder of pS. The endoprotease requires sulfhydryl groups for its activity and is present in a C. reinhardtii postribosomal supernatant as well as in a free polysome fraction. The latter could account for the observation that completion of nascent chains in free polysomes yielded S but not pS. We propose that pS is an extacloroplastic form of S and that the smallfpeptide portion plays a role in the transfer of S into the chloroplast.
abstract_translated_lang: eng
date: 1977
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008345
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 005
ppn_swb: 1349105724
own_urn: urn:nbn:de:bsz:16-opus-83459
language: ger
bibsort: DOBBERSTEIINVITROSYN1977
full_text_status: public
publication: Proc. Natl. Acad. Sci. USA
volume: 74
number: 3
pagerange: 1082-1085
citation:   Dobberstein, Bernhard ; Blobel, Günter ; Chua, Nam-Hai  (1977) In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii.  Proc. Natl. Acad. Sci. USA, 74 (3).  pp. 1082-1085.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8345/1/Dobberstein_1977_PNAS.pdf