title: Biophysical Characterization and Simulation of Neocortical Layer 2/3 Pyramidal Neurons during Postnatal Development
creator: Fucke, Thomas
subject: 570
subject: 570 Life sciences
description: Pyramidal neurons in layer 2/3 of the mammalian neocortex constitute the most abundant neocortical cell type, yet their biophysical properties are still poorly understood. In this thesis, fundamental properties of layer 2/3 pyramidal neurons of 1-to-6-weeks old rats were investigated with an approach combining in vitro electrophysiological characterization, reconstruction of cell morphologies, and numerical computer simulations. A specific goal was to identify ion channel mechanisms underlying the sub-threshold integrative properties of these cells and to reveal the developmental profile of channel expression. A simulated annealing algorithm was employed to numerically simulate layer 2/3 neurons and to generate valid models of varying complexity and constrained by experimental data. At all ages, layer 2/3 pyramidal neurons showed prominent anomalous rectification which could be attributed to inward-rectifier potassium (KIR) channels based both on pharmacological experiments and modeling. In contrast to other types of pyramidal neurons little hyperpolarization-activated current (Ih) was found. While morphological development essentially was complete at postnatal week 2, biophysical properties continued to change until week 4-6. In particular, input resistance strongly decreased with age, rendering the cells less excitable as the cortical network matures. Computer simulations showed that these properties will have a large impact on the integration of synaptic inputs during ongoing spontaneous activity in vivo. It is concluded, that layer 2/3 pyramidal neurons possess biophysical properties distinct from other pyramidal cells and that the prolonged postnatal development is critical for shaping synaptic integration and neocortical circuit activity in vivo.
date: 2007
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8349/1/Dissertation_ThomasFucke.pdf
identifier: DOI:10.11588/heidok.00008349
identifier: urn:nbn:de:bsz:16-opus-83492
identifier:   Fucke, Thomas  (2007) Biophysical Characterization and Simulation of Neocortical Layer 2/3 Pyramidal Neurons during Postnatal Development.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8349/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: ger