TY - JOUR UR - https://archiv.ub.uni-heidelberg.de/volltextserver/8378/ EP - 2158 IS - 9 AV - public KW - in vitro transcription-translation KW - zein mRNA KW - inverted repeats KW - 5'- and 3'-untranslated regions KW - translational potential JF - The EMBO Journal ID - heidok8378 TI - Translation efficiency of zein mRNA is reduced by hybrid formation between the 5´-and 3´-untranslated region Y1 - 1985/// VL - 4 SP - 2153 N2 - The secondary structure of zein mRNA affects its translational potential. Here we show that in a cell-free system the translation efficiency of zein mRNA containing inverted repeats in the 5'- and 3'-untranslated regions is reduced. This translational block is released after deletion of the 3'-inverted repeat. We conclude that the translational block is caused by hybrid formation between the two inverted repeats. The translational efficiency of zein mRNAs, is also affected by varying the length or the primary structure of the 5'-untranslated region. A1 - Spena, A. A1 - Krause, E. A1 - Dobberstein, Bernhard ER -