eprintid: 8378
rev_number: 7
eprint_status: archive
userid: 1
dir: disk0/00/00/83/78
datestamp: 2008-04-30 15:26:13
lastmod: 2014-04-03 20:44:38
status_changed: 2012-08-14 15:25:16
type: article
metadata_visibility: show
creators_name: Spena, A.
creators_name: Krause, E.
creators_name: Dobberstein, Bernhard
title: Translation efficiency of zein mRNA is reduced by hybrid formation between the 5´-and 3´-untranslated region
ispublished: pub
subjects: ddc-570
divisions: i-706000
keywords: in vitro transcription-translation , zein mRNA , inverted repeats , 5'- and 3'-untranslated regions , translational potential
abstract: The secondary structure of zein mRNA affects its translational potential. Here we show that in a cell-free system the translation efficiency of zein mRNA containing inverted repeats in the 5'- and 3'-untranslated regions is reduced. This translational block is released after deletion of the 3'-inverted repeat. We conclude that the translational block is caused by hybrid formation between the two inverted repeats. The translational efficiency of zein mRNAs, is also affected by varying the length or the primary structure of the 5'-untranslated region.
abstract_translated_lang: eng
date: 1985
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008378
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 030
ppn_swb: 1349723487
own_urn: urn:nbn:de:bsz:16-opus-83784
language: eng
bibsort: SPENAATRANSLATIO1985
full_text_status: public
publication: The EMBO Journal 
volume: 4
number: 9
pagerange: 2153-2158
citation:   Spena, A. ; Krause, E. ; Dobberstein, Bernhard  (1985) Translation efficiency of zein mRNA is reduced by hybrid formation between the 5´-and 3´-untranslated region.  The EMBO Journal , 4 (9).  pp. 2153-2158.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8378/1/Spena_1985_EMBOJ.pdf