title: Digital In-Line X-Ray Holographic Microscopy with Synchrotron Radiation
creator: Barth, Ruth
subject: ddc-530
subject: 530 Physics
description: In-line holography is a lensless microscopy method yielding three-dimensional images of the object, where the resolution limit depends on the illuminating wavelength and the numerical aperture of the system, only. In this work, an in-line holographic setup was implemented with radiation in the vacuum-ultraviolet and soft X-ray region. Diverging wavefronts were generated by diffraction of the synchrotron radiation from a pinhole with suitable diameter. The holographic interference pattern was recorded with a CCD camera, and the images were numerically reconstructed using the Kreuzer implementation of the Kirchhoff-Helmholtz transform. With this microscopic technique, lithographic structures in photo resist, mixtures of polystyrene beads and iron oxide particles, and dried biological samples such as rat embryonic fibroblast cells, green algae Ulva linza, and human chromosomes were imaged. The achieved resolution was determined via different criteria and was compared to the theoretical expectations. It was shown, that by increasing the numerical aperture with smaller pinholes and by correcting for drift effects, the experimental resolution could be improved from delta(exp) = 1.13 +- 0.35 µm to delta(exp) = 0.37 +- 0.04 µm, meeting the theoretical prediction of delta(theo) = 0.34 µm. Furthermore, by recording holograms at different energies, element contrast was obtained.
date: 2008
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8467/1/Dissertation_Ruth_Barth.pdf
identifier: DOI:10.11588/heidok.00008467
identifier: urn:nbn:de:bsz:16-opus-84671
identifier:   Barth, Ruth  (2008) Digital In-Line X-Ray Holographic Microscopy with Synchrotron Radiation.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8467/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng