eprintid: 8473
rev_number: 8
eprint_status: archive
userid: 1
dir: disk0/00/00/84/73
datestamp: 2008-06-17 15:16:29
lastmod: 2014-08-18 09:50:20
status_changed: 2012-08-14 15:25:35
type: article
metadata_visibility: show
creators_name: Haeuptle, Marie-Theres
creators_name: Flint, Nicholas
creators_name: Gough, Nicholas M.
creators_name: Dobberstein, Bernhard
title: A Tripartite Structure of the Signals that Determine Protein Insertion into the Endoplasmic Reticulum Membrane
ispublished: pub
subjects: ddc-570
divisions: i-706000
abstract: Multilineage colony stimulating factor is a secretory protein with a cleavable signal sequence that is unusually long and hydrophobic. Using molecular cloning techniques we exchanged sequences NH2- or COOH-terminally flanking the hydrophobic signal sequence. Such modified fusion proteins still inserted into the membrane but their signal sequence was not cleaved. Instead the proteins were now anchored in the membrane by the formerly cleaved signal sequence (signal-anchor sequence). They exposed the NH2 terminus on the exoplasmic and the COOH terminus on the cytoplasmic side of the membrane. We conclude from our results that hydrophilic sequences flanking the hydrophobic core of a signal sequence can determine cleavage by signal peptidase and insertion into the membrane. It appears that negatively charged amino acid residues close to the NH2 terminal side of the hydrophobic segment are compatible with translocation of this segment across the membrane. A tripartite structure is proposed for signal-anchor sequences: a hydrophobic core region that mediates targeting to and insertion into the ER membrane and flanking hydrophilic segments that determine the orientation of the protein in the membrane.
abstract_translated_lang: eng
date: 1989
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008473
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 044
ppn_swb: 1352624478
own_urn: urn:nbn:de:bsz:16-opus-84737
language: eng
bibsort: HAEUPTLEMAATRIPARTIT1989
full_text_status: public
publication: The Journal of Cell Biology
volume: 108
pagerange: 1227-1236
citation:   Haeuptle, Marie-Theres ; Flint, Nicholas ; Gough, Nicholas M. ; Dobberstein, Bernhard  (1989) A Tripartite Structure of the Signals that Determine Protein Insertion into the Endoplasmic Reticulum Membrane.  The Journal of Cell Biology, 108.  pp. 1227-1236.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8473/1/Haeuptle_1989_JCB.pdf