<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents"^^ . "Bifunctional cross-linking reagents were used to probe the protein environment in the ER membrane of the signal sequence receptor (SSR), a 34-kD integral membrane glycoprotein (Wiedmann, M., T. V. Kurzchalia, E. Hartmarm, and T. A. Rapoport. 1987. Nature [Lond.]. 328:830-833). The proximity of several polypeptides was demonstrated. A 22-kD glycoprotein was identified tightly bound to the 34-kD SSR even after membrane solubilization. The 34-kD polypeptide, now termed otSSR, and the 22-kD polypeptide, the #SSR, represent a heterodimer. We report on the sequence of the/3SSR, its membrane topology, and on the mechanism of its integration into the membrane. Cross-linking also produced dimers of the a-subunit of the SSR indicating that oligomers of the SSR exist in the ER membrane. Various bifunctional cross-linking reagents were used to study the relation to ER membrane proteins of nascent chains of preprolactin and/3-1actamase at different stages of their translocation through the membrane. The predominant cross-linked products obtained in high yields contained the aSSR, indicating in conjunction with previous results that it is a major membrane protein in the neighborhood of translocating nascent chains of secretory proteins. The results support the existence of a translocon, a translocation complex involving the SSR, which constitutes the specific site of protein translocation across the ER membrane."^^ . "1990" . . . "111" . "6" . . "The Journal of Cell Biology"^^ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Tom A."^^ . "Rapoport"^^ . "Tom A. Rapoport"^^ . . "Enno"^^ . "Hartmann"^^ . "Enno Hartmann"^^ . . "Siegne"^^ . "Knespel"^^ . "Siegne Knespel"^^ . . "Joachim"^^ . "Herz"^^ . "Joachim Herz"^^ . . "Regine"^^ . "Kraft"^^ . "Regine Kraft"^^ . . "Siegfried"^^ . "Prehn"^^ . "Siegfried Prehn"^^ . . "Albrecht"^^ . "Otto"^^ . "Albrecht Otto"^^ . . "Dirk"^^ . "Görlich"^^ . "Dirk Görlich"^^ . . "Bernhard"^^ . "Dobberstein"^^ . "Bernhard Dobberstein"^^ . . "Martin"^^ . "Wiedmann"^^ . "Martin Wiedmann"^^ . . . . . . "The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents (PDF)"^^ . . . "Goerlich_1990_JCB.pdf"^^ . . . "The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents (Other)"^^ . . . . . . "preview.jpg"^^ . . . "The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents (Other)"^^ . . . . . . "medium.jpg"^^ . . . "The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #8476 \n\nThe Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .