title: A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins
creator: Hauser, Stefanie
creator: Bacher, Gerald
creator: Dobberstein, Bernhard
creator: Lütcke, Henrich
subject: 570
subject: 570 Life sciences
description: Translocation of proteins across the endoplasmic reticulum membrane is initiated by the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex consisting of a 7S RNA and six polypeptides. To investigate the functions of the SRP components, we have tested the activities of several SRP subparticles. We show that the SRP GTPase (SRP54) alone binds a signal sequence and discriminates it from a non-signal sequence. Although SRP54 alone is unable to promote translocation, SRP54 in a complex with SRP RNA is both necessary and sufficient to promote translocation of an elongation-arrested nascent protein in a GTPregulated manner. For co-translational translocation, additional SRP components are required. We discuss the implications of our results for the function of the Escherichia coli SRP which is homologous to the SRP54/SRP-RNA complex. 
date: 1995
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8478/1/Hauser_1995_EMBOJ.pdf
identifier: DOI:10.11588/heidok.00008478
identifier: urn:nbn:de:bsz:16-opus-84780
identifier:   Hauser, Stefanie ; Bacher, Gerald ; Dobberstein, Bernhard ; Lütcke, Henrich  (1995) A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins.  The EMBO Journal, 14 (22).  pp. 5485-5493.      
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8478/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: ger