TY  - JOUR
KW  - endoplasmic reticulum 
KW  -  protein translocation 
KW  -  signal recognition particle (SRP) 
KW  -  signal sequence recognition 
KW  -  4.5S RNA
IS  - 22
Y1  - 1995///
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/8478/
N2  - Translocation of proteins across the endoplasmic reticulum membrane is initiated by the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex consisting of a 7S RNA and six polypeptides. To investigate the functions of the SRP components, we have tested the activities of several SRP subparticles. We show that the SRP GTPase (SRP54) alone binds a signal sequence and discriminates it from a non-signal sequence. Although SRP54 alone is unable to promote translocation, SRP54 in a complex with SRP RNA is both necessary and sufficient to promote translocation of an elongation-arrested nascent protein in a GTPregulated manner. For co-translational translocation, additional SRP components are required. We discuss the implications of our results for the function of the Escherichia coli SRP which is homologous to the SRP54/SRP-RNA complex. 
A1  - Hauser, Stefanie
A1  - Bacher, Gerald
A1  - Dobberstein, Bernhard
A1  - Lütcke, Henrich
JF  - The EMBO Journal
AV  - public
ID  - heidok8478
TI  - A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins
SP  - 5485
EP  - 5493
VL  - 14
ER  -