eprintid: 8478
rev_number: 8
eprint_status: archive
userid: 1
dir: disk0/00/00/84/78
datestamp: 2008-06-17 17:33:56
lastmod: 2014-08-21 00:16:25
status_changed: 2012-08-14 15:25:37
type: article
metadata_visibility: show
creators_name: Hauser, Stefanie
creators_name: Bacher, Gerald
creators_name: Dobberstein, Bernhard
creators_name: Lütcke, Henrich
title: A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins
ispublished: pub
subjects: 570
divisions: 706000
keywords: endoplasmic reticulum , protein translocation , signal recognition particle (SRP) , signal sequence recognition , 4.5S RNA
abstract: Translocation of proteins across the endoplasmic reticulum membrane is initiated by the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex consisting of a 7S RNA and six polypeptides. To investigate the functions of the SRP components, we have tested the activities of several SRP subparticles. We show that the SRP GTPase (SRP54) alone binds a signal sequence and discriminates it from a non-signal sequence. Although SRP54 alone is unable to promote translocation, SRP54 in a complex with SRP RNA is both necessary and sufficient to promote translocation of an elongation-arrested nascent protein in a GTPregulated manner. For co-translational translocation, additional SRP components are required. We discuss the implications of our results for the function of the Escherichia coli SRP which is homologous to the SRP54/SRP-RNA complex. 
abstract_translated_lang: eng
date: 1995
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008478
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 075
ppn_swb: 1352586894
own_urn: urn:nbn:de:bsz:16-opus-84780
language: ger
bibsort: HAUSERSTEFACOMPLEXOF1995
full_text_status: public
publication: The EMBO Journal
volume: 14
number: 22
pagerange: 5485-5493
citation:   Hauser, Stefanie ; Bacher, Gerald ; Dobberstein, Bernhard ; Lütcke, Henrich  (1995) A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins.  The EMBO Journal, 14 (22).  pp. 5485-5493.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8478/1/Hauser_1995_EMBOJ.pdf