eprintid: 8480
rev_number: 16
eprint_status: archive
userid: 1
dir: disk0/00/00/84/80
datestamp: 2008-06-17 17:33:01
lastmod: 2016-01-19 00:41:04
status_changed: 2012-08-14 15:25:36
type: article
metadata_visibility: show
creators_name: Meyer, David I.
creators_name: Dobberstein, Bernhard
title: A Membrane Component Essential for Vectorial Translocation of Nascent Proteins across the Endoplasmic Reticulum: Requirements for Its Extraction and Reassociation with the Membrane
ispublished: pub
subjects: ddc-570
divisions: i-706000
abstract: Previous reports have shown that rough microsomes treated with high salt (Warren and Dobberstein, 1978, Nature, 273:569-571) or proteases (Walter et al ., 1979, Proc. Nati. Acad. Sci. U. S. A., 76:1,795) are unable to vectorially translocate nascent proteins. Readdition of the high salt or protease extracts restored activity to such inactive rough microsomes. A detailed study was carried out to determine how this factor interacts with the rough microsomal membrane. Proteolytic cleavage was found to be necessary but not sufficient to remove this factor from the membrane. A subsequent treatment with high salt had to be carried out . Endogenous (pancreatic) protease could effect the required cleavage, but low levels of trypsin, clostripain, or elastase were far more efficient. Several proteases were not effective. The minimum level of salt (after proteolysis) required to solubilize the active factor was -200 mM KCI. Salt extracts prepared by treatment with one of the effective proteases were capable of restoring activity to inactive microsomes produced by treatment with one of the others .
abstract_translated_lang: eng
date: 1980
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008480
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 011
ppn_swb: 135258820X
own_urn: urn:nbn:de:bsz:16-opus-84803
language: eng
bibsort: MEYERDAVIDAMEMBRANEC1980
full_text_status: public
publication: THE JOURNAL OF CELL BIOLOGY
volume: 87
pagerange: 498-502
citation:   Meyer, David I. ; Dobberstein, Bernhard  (1980) A Membrane Component Essential for Vectorial Translocation of Nascent Proteins across the Endoplasmic Reticulum: Requirements for Its Extraction and Reassociation with the Membrane.  THE JOURNAL OF CELL BIOLOGY, 87.  pp. 498-502.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8480/1/Meyer_1980a_JCB.pdf