title: Identification and Characterization of a Membrane Component Essential for the Translocation of Nascent Proteins across the Membrane of the Endoplasmic Reticulum
creator: Meyer, David I.
creator: Dobberstein, Bernhard
subject: ddc-570
subject: 570 Life sciences
description: When rough microsomes are subjected to limited proteolysis and high salt, a soluble fraction can be separated from the membrane . Neither fraction alone is capable of vectorially translocating nascent peptides . When the soluble extract is recombined with the residual membrane fraction, translocating activity is restored . Standard biochemical techniques were used to identify and characterize the active component derived by treating rough microsomes with elastase and high salt. The active factor is a peptide fragment with an apparent molecular weight of 60,000. It represents the cytoplasmic domain of a larger membrane protein. The fragment is basic and has at least one accessible sulfhydryl group. These characteristics facilitated its purification and identification as a membrane component required for translocation of nascent peptides across microsomal membranes.
date: 1980
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8481/1/Meyer_1980b_JCB.pdf
identifier: DOI:10.11588/heidok.00008481
identifier: urn:nbn:de:bsz:16-opus-84810
identifier:   Meyer, David I. ; Dobberstein, Bernhard  (1980) Identification and Characterization of a Membrane Component Essential for the Translocation of Nascent Proteins across the Membrane of the Endoplasmic Reticulum.  THE JOURNAL OF CELL BIOLOGY , 87.  pp. 503-508.      
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8481/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng