eprintid: 8550
rev_number: 8
eprint_status: archive
userid: 1
dir: disk0/00/00/85/50
datestamp: 2008-07-15 17:47:19
lastmod: 2014-08-25 04:40:54
status_changed: 2012-08-14 15:25:53
type: article
metadata_visibility: show
creators_name: Lipp, Joachim
creators_name: Flint, Nicholas
creators_name: Haeuptle, Marie-Theres
creators_name: Dobberstein, Bernhard
title: Structural Requirements for Membrane Assembly of Proteins Spanning the Membrane Several Times
ispublished: pub
subjects: ddc-570
divisions: i-706000
abstract: We have investigated the structural requirements for the biogenesis of proteins spanning the membrane several times. Proteins containing various combinations of topological signals (signal anchor and stop transfer sequences) were synthesized in a cell-free translation system and their membrane topology was determined. Proteins spanning the membrane twice were obtained when a signal anchor sequence was followed by either a stop transfer sequence or a second signal anchor sequence.  Thus, a signal anchor sequence in the second position can function as a stop transfer sequence, spanning the membrane in the opposite orientation to that of the first signal anchor sequence. A signal anchor sequence in the third position was able to insert amino acid sequences located COOH terminal to it. We conclude that proteins spanning the membrane several times can be generated by stringing together signal anchor and stop transfer sequences. However, not all proteins with three topological signals were found to span the membrane three times. A certain segment located between the first and second topological signal could prevent stable membrane integration of a third signal anchor segment.
abstract_translated_lang: eng
date: 1989
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008550
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 046
ppn_swb: 135403709X
own_urn: urn:nbn:de:bsz:16-opus-85508
language: eng
bibsort: LIPPJOACHISTRUCTURAL1989
full_text_status: public
publication: The Journal of Cell Biology
volume: 109
pagerange: 2013-2022
citation:   Lipp, Joachim ; Flint, Nicholas ; Haeuptle, Marie-Theres ; Dobberstein, Bernhard  (1989) Structural Requirements for Membrane Assembly of Proteins Spanning the Membrane Several Times.  The Journal of Cell Biology, 109.  pp. 2013-2022.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8550/1/Lipp_1989_JCB.pdf